text: Carnitine biosynthesis. Purification of gamma-butyrobetaine hydroxylase from rat liver.
  gamma-Butyrobetaine hydroxylase catalyse the last step in carnitine biosynthesis, the formation of L-carnitine from gamma-butyrobetaine, a reaction dependent on Fe2+, alpha-ketoglutarate, ascorbate and oxygen. Initial attempts to purify the protein from rat liver showed that gamma-butyrobetaine hydroxylase is unstable. We, therefore, determined the influence of various compounds on the stability of gamma-butyrobetaine hydroxylase at different storage temperatures. The enzyme activity was best conserved by storing the protein at 4 degrees C in the presence of 200 g/l glycerol and 10 mM DTT. We subsequently purified the enzyme from rat liver to apparent homogeneity by liquid chromatography.
entities.id: T10 T11 T12 T13 T14 T15 T16 T17 T18 T1 T2 T3 T4 T5 T6 T7 T8 T9
entities.type: CHEMICAL CHEMICAL CHEMICAL CHEMICAL CHEMICAL GENE-Y GENE-Y GENE-Y GENE-Y CHEMICAL CHEMICAL CHEMICAL CHEMICAL CHEMICAL CHEMICAL CHEMICAL CHEMICAL CHEMICAL
entities.text: carnitine DTT L-carnitine Carnitine gamma-butyrobetaine gamma-Butyrobetaine hydroxylase gamma-butyrobetaine hydroxylase gamma-butyrobetaine hydroxylase gamma-butyrobetaine hydroxylase gamma-Butyrobetaine gamma-butyrobetaine Fe2+ alpha-ketoglutarate ascorbate oxygen gamma-butyrobetaine gamma-butyrobetaine glycerol