#app.R library(shiny) library(BioAge) library(dplyr) #library(RSQLite) library(googlesheets4) library(googledrive) library(shinyvalidate) library(shinyjs) library(plotly) library(lubridate) library(pander) library(tidyr) library(shiny.router) #options(gargle_oauth_cache = ".secrets") createReactiveDataset <- function() { return(reactiveVal(data.frame(date = as.Date(character()), quantity = integer(), item = character()))) } # Define dataset and its reactive variable dataset <- createReactiveDataset() # Dummy dataset for development localDataset <- reactiveVal(data.frame( date = as.Date(c('2024-12-01', '2024-12-02')), quantity = c(10, 20), item = c('Apples', 'Oranges') )) # For local development, bypass authentication isLocal <- TRUE home_route <- route("/", function() { fluidPage( h1("Home Page"), DT::dataTableOutput("dataTable") ) }) checkRange <- function(value, min, max){ if(!is.na(value) & (value < min || value > max)){ paste0("Please specify a number that is between ", min, " and ", max, ", thank you!") } else { "" } } search_string = "" query_params <- parseQueryString(search_string) #if !is.null() emiglio = query_params$useremail nomignolo = paste0(query_params$firstname, " ", query_params$lastname) phone_prefix_regex <- "^\\+?\\d{1,3}$" phone_number_regex <- "^[0-9]{10,10}$" validatePhone <- function(value, n_char_min){ if(value != ""){ if(grepl("^[0-9]{1,100}$", value)){ if (nchar(value) == n_char_min) { "" } else{ paste0("Please specify a number that contains ", n_char_min, " digits") } }else{ "Please enter only digits" } } else{ "" } } ui <- fluidPage( tags$head( tags$link(rel = "stylesheet", href = "https://cdnjs.cloudflare.com/ajax/libs/font-awesome/6.0.0-beta3/css/all.min.css"), tags$style(HTML(" .control-label { color: black !important; } .strongy{ background-color: #c8e6c9; padding: 10px } .form-control{ border-color: grey !important } #loading { display: none; text-align: center; } .loader { border: 6px solid #f3f3f3; /* Light grey */ border-top: 6px solid #3498db; /* Blue */ border-radius: 50%; width: 50px; height: 50px; animation: spin 2s linear infinite; margin: 20px auto; } @keyframes spin { 0% { transform: rotate(0deg); } 100% { transform: rotate(360deg); } } ")) ), useShinyjs(), titlePanel("Biological Age Calculator"), sidebarLayout( sidebarPanel( fluidRow( column(6, textInput("Name", "Enter name", query_params$firstname)), column(6, textInput("Surname", "Enter your last name", query_params$lastname)) ), fluidRow( column(6, textInput("phone_prefix", "Enter Country Code", "+91")), column(6, textInput("phone_number", "Enter your phone number", "")) ), dateInput("dob", "Date of Birth*", value="1990-01-01"), dateInput("bloodTestDate", "Date of TEST", value = Sys.Date()), numericInput("albumin", "Albumin (g/dL)", value = NA, min = 0), verbatimTextOutput("values"), numericInput("lymph", "Lymphocytes (%)", value = NA, min = 0), numericInput("mcv", "Mean Cell Volume (MCV)", value = NA, min = 0), numericInput("glucose", "Glucose (mg/L)", value = NA, min = 0), numericInput("rdw", "Red Cell Dist Width (RDW)", value = NA, min = 0), numericInput("creat", "Creatinine (mg/dL)", value = NA, min = 0), numericInput("crp", "CRP (mg/L)", value = NA, min = 0), numericInput("alp", "Alkaline Phosphatase (U/L)", value = NA, min = 0), numericInput("wbc", "White Blood Cells (cells/mL)", value = NA, min = 0), actionButton("submit", "Submit", title = "Fill in the phone number field to enable") ), mainPanel( tabsetPanel( tabPanel("Calculator", div(id = "loading", class = "loader", style = "display: none"), # Loading spinner uiOutput("results"), uiOutput("message")), tabPanel("My historical data - Plot", uiOutput("notlogged"), actionLink("openLinkButton", "Create an account here / log in here", href = "", style = "background-color:#c8e6c9; padding: 10px; font-size: 2em"), plotOutput("biologicalAgePlot")) , tabPanel("My historical data - Table", tableOutput("table"), actionLink("openLinkButton", "Create an account here / log in here", href = "", style = "background-color:#c8e6c9; padding: 10px; font-size: 2em"), uiOutput("notlogged1")) ) ) ) ) server <- function(input, output, session) { sheet_id <- "https://docs.google.com/spreadsheets/d/1xQpghper_5FCWkYFByIpdVKFmBofgM7uVrG_bsaGW58/edit?gid=0#gid=0" values <- reactiveVal(data.frame()) router_server( list(home_route) ) # Dummy dataset #dataset <- data.frame(date = as.Date(c('2024-12-01', '2024-12-02')), #quantity = c(10, 20), #item = c('Apples', 'Oranges')) # Render the dataset in a table #output$dataTable <- DT::renderDataTable({ #dataset #}) observeEvent(input$submit, { shinyjs::show(id = "loading") # Show loading spinner # Collecting inputs name <- paste0(input$Name, " ", input$Surname) ageAtTestDate <- round(as.numeric(difftime(Sys.Date(), input$dob, units = "days")) / 365.25, 1) biological_age <- runif(1, min = ageAtTestDate - 5, max = ageAtTestDate + 5) # Simulated calculation bioage_color <- if (biological_age < ageAtTestDate) "green" else if (biological_age > ageAtTestDate) "red" else "yellow" # Show the BioAge Modal show_bioage_modal(name, ageAtTestDate, biological_age, bioage_color) output$results <- renderUI({ paste("Thank you for submitting your details. Your biological age will be calculated based on the provided data.") }) }) # Define show_bioage_modal function outside observeEvent show_bioage_modal <- function(name, ageAtTestDate, biological_age, bioage_color) { diff <- round(abs(biological_age - ageAtTestDate), 2) message <- if (biological_age < ageAtTestDate) { tags$p( paste0( name,", your healthy choices show! your BioAge is ", diff, " years younger than your calendar Age." ), style = "padding-bottom: 24px; font-size: 16px; font-weight: bold; font-family: sans-serif; font-variant: contextual;" ) } else if (biological_age > ageAtTestDate) { tags$p( paste0( name,", your BioAge is ", diff, " years higher than your Calendar Age. Don’t worry—small, consistent steps in the right direction can help you close the gap!" ), style = "padding-bottom: 24px; font-size: 16px; font-weight: bold; font-family: sans-serif; font-variant: contextual;" ) } else { tags$p( paste0( name,", your BioAge matches your Calendar Age, indicating balanced health markers." ), style = "padding-bottom: 24px; font-size: 16px; font-weight: bold; font-family: sans-serif; font-variant: contextual;" ) } showModal( modalDialog( title = NULL, div( style = "background-color: #0E406B; color: white; padding: 24px; text-align: center; border-top-left-radius: 5px; border-top-right-radius: 5px; margin: -15px -15px 0 -15px; font-family: 'Archia', sans-serif;", h3( paste0(name,"'s BioAge Results"), style = "margin: 0; padding: 0; font-size: 26px; text-align: center; font-family: 'Archia', sans-serif; font-variant: common-ligatures; color: white; line-height: 1;" ) ), div( style = "background-color: white; text-align: center; font-family: 'Archia', sans-serif; padding-top: 20px;", fluidRow( column(5, div( tags$p("Calendar Age", style = "font-weight: bold; font-size: 20px; color: black;"), tags$p(paste(ageAtTestDate, "years"), style = "font-size: 24px; font-weight: bold; color: black;") )), column(2, div( tags$p("→", style = "font-size: 59px; font-weight: bold; color: darkblue; margin-top: 8px;") )), column(5, div( tags$p("BioAge", style = "font-weight: bold; font-size: 20px; color: black;"), tags$p( paste(round(biological_age, 2), "years"), style = sprintf("font-size: 24px; font-weight: bold; color: %s;", bioage_color) ) )) ) ), div( style = "text-align: center; margin-top: 20px; color: #333333; margin-bottom: 0px;", message ), div( style = "text-align: center;", tags$p("Complete report sent to your WhatsApp", tags$i(class = "fa-brands fa-whatsapp fa-beat", style = "color: #25D366; margin-left: 5px; font-size: 16px;"), style = "font-size: 14px; color: black; margin-bottom: 0px;"), tags$p("xxxxxxxx4321", style = "font-size: 14px; color: black; margin-bottom: -16px;") ), easyClose = FALSE, footer = tags$button( "Close", onclick = "Shiny.setInputValue('close_modal', true);", style = "background-color: #86D1F1; color: black; border: none; padding: 10px 20px; border-radius: 5px; font-size: 16px; cursor: pointer;" ) ) ) } output$message <- renderUI({ HTML("

What is Biological Age?

Biological age refers to how old a person seems based on the functioning and condition of their body systems, rather than the time since birth (chronological age). It is influenced by genetics, lifestyle, and environmental factors, and can provide a more accurate representation of an individual's health and longevity prospects.

Our tool can estimate your biological age using even one blood test parameter. However, for the most accurate results, we recommend including as many parameters as possible.

Here is what each of the parameters mean for your health:

  1. Albumin: A protein in the blood that helps maintain fluid balance and transport hormones, vitamins, and drugs; low levels can indicate liver or kidney disease.
  2. Creatinine: A waste product from muscle metabolism; its blood level is a marker of kidney function, with high levels indicating potential kidney impairment.
  3. Glucose: The main sugar found in the blood and the body's primary energy source; levels are used to diagnose and monitor diabetes.
  4. C-reactive Protein (CRP): A substance produced by the liver in response to inflammation; high CRP levels can indicate infection or chronic inflammatory diseases.
  5. Lymphocyte (Lymphs): A type of white blood cell involved in the immune response; changes in lymphocyte levels can indicate infections, autoimmune diseases, or blood disorders.
  6. Mean Cell Volume (MCV): The average size of red blood cells; it helps diagnose and classify anemias, with high MCV indicating macrocytic anemia and low MCV indicating microcytic anemia.
  7. Red Cell Distribution Width (RDW): A measure of the variation in red blood cell size; increased RDW can indicate anemia and has been associated with cardiovascular diseases.
  8. Alkaline Phosphatase: An enzyme found in the liver, bones, and other tissues; high levels can indicate liver disease, bone disorders, or bile duct obstruction.
  9. White Blood Cells (WBCs): Cells in the immune system that help defend against infections; abnormal levels can indicate infection, inflammation, or immune system disorders.

Based on scientific data:

We have based this calculation of your biological age on scientific data from the National Health and Nutrition Examination Survey (NHANES). The package uses published biomarker algorithms to calculate three biological aging measures: Klemera-Doubal Method (KDM) biological age, phenotypic age, and homeostatic dysregulation.

Citation:

Kwon, D., Belsky, D.W. A toolkit for quantification of biological age from blood chemistry and organ function test data: BioAge. GeroScience 43, 2795–2808 (2021).

Disclaimer:

These results are solely for informational use and shouldn't replace professional medical advice, diagnosis, or treatment. Always seek the guidance of a healthcare provider for any medical conditions or before making changes to your health regimen, including starting new diets, exercises, or supplements, or altering medication. Never discontinue medication or follow any health advice without consulting your healthcare provider.

") }) observeEvent(input$submit, { # Handle form submission output$results <- renderUI({ paste("Thank you for submitting your details. Your biological age will be calculated based on the provided data.") }) }) output$notlogged <- renderUI({ if (is.null(emiglio)){ HTML("

Please log in to View your history

") } else { "" } }) output$notlogged1 <- renderUI({ if (is.null(emiglio)){ HTML("

Please log in to View your history

") } else { "" } }) output$biologicalAgePlot <- renderPlot( if (!is.null(emiglio)){ if(nrow(values %>% filter(email == emiglio))>0){ values %>% filter(email == emiglio) %>% select(c(age, age_bio, date)) %>% group_by(date) %>% mutate(age = mean(age, na.rm = T), age_bio = mean(age_bio, na.rm = T)) %>% rename( `Actual age` = age, `Biological age` = age_bio ) %>% mutate(Date = as_date(date)) %>% pivot_longer(cols = c(1:2), names_to = "name", values_to = "value") %>% ggplot(aes( x = Date, y = value, color = name ) )+ geom_line(size = 1.3)+ theme_bw()+ scale_color_discrete(name = "")+ scale_y_continuous(breaks = round(seq(min(c(values$age, values$age_bio)), max(c(values$age, values$age_bio)), by = 2),0)) } } ) output$table <- renderTable( if (!is.null(emiglio)){ if(nrow(values %>% filter(email == emiglio))>0){ values %>% filter(email == emiglio) %>% mutate_at(c(1, 11), round, 1) %>% select(c(15, 2:10, 1, 11)) %>% #arrange(-date) %>% mutate(date = as.character(as.Date(date)))%>% rename( Creatinine = creat, `Lymphocyte (Lymphs)` = lymph, `CRP (C-reactive Protein)` = crp, `White Blood Cells` = wbc, `Mean Cell Volume` = mcv, `Red cells distribution width` = rdw, Glucose = glucose, `Alkaline Phosphatase` = alp, `Actual Age` = age, `Biological Age` = age_bio ) } } ) observe({ iv <- InputValidator$new() iv$add_rule("albumin", checkRange, 2, 8) iv$add_rule("creat", checkRange, .2, 2) iv$add_rule("rdw", checkRange, 10, 20) iv$add_rule("crp", checkRange, 0, 50) iv$add_rule("lymph", checkRange, 0, 100) iv$add_rule("glucose", checkRange, 40, 400) iv$add_rule("wbc", checkRange, 2, 25) iv$add_rule("alp", checkRange, 0, 300) iv$add_rule("mcv", checkRange, 70, 120) iv$add_rule("phone_number", validatePhone, 10) iv$enable() output$values <- renderPrint({ req(iv$is_valid()) }) }) observeEvent(input$submit, { shinyjs::show(id = "loading") # Show loading spinner nome = paste0(input$Name, " ", input$Surname) ageAtTestDate <- round(as.numeric(difftime(Sys.Date(), input$dob, units = "days")) / 365.25, 1) if (!is.null(input$dob)) { allInputs <- data.frame( age = as.numeric(difftime(Sys.Date(), input$dob, units = "days")) / 365.25, albumin = input$albumin, lymph = input$lymph, mcv = input$mcv, glucose = input$glucose, rdw = input$rdw, creat = input$creat, crp = input$crp, alp = input$alp, wbc = input$wbc ) } if (!is.null(input$phone_number) && !is.null(phone_number_regex) && grepl(phone_prefix_regex, input$phone_prefix) && grepl(phone_number_regex, input$phone_number)) { validated_phone <- paste(input$phone_prefix, input$phone_number) } else { validated_phone = NA } mrkrs = colnames(allInputs[, colSums(is.na(allInputs)) < nrow(allInputs)]) train = phenoage_calc(NHANES3, biomarkers = mrkrs) biological_age <- phenoage_calc(allInputs, biomarkers = mrkrs, fit = train$fit)$data$phenoage allInputs_tosave = allInputs %>% mutate( age_bio = biological_age, email = ifelse(is.null(emiglio), "", emiglio), name = nome, phone = validated_phone, date= input$bloodTestDate ) biologicalAge <- if (!is.null(input$dob)) { paste("Your biological age is approximately:", round(biological_age, 1), "years") } else { "Please enter at least your date of birth" } observeEvent(input$close_modal, { removeModal() }) output$results <- renderUI( HTML( paste0( "
", "

Hello ", nome, "

", "

Your biological age is ", round(biological_age, 1), " years

", "

Your actual age is ", ageAtTestDate, " years

" ) ) ) # Append data to Google Sheet if (nrow(allInputs_tosave) == 1) { if (nrow(values()) == 0) { sheet_write(data = allInputs_tosave, ss = sheet_id, sheet = "longevity") } else { sheet_append(data = allInputs_tosave, ss = sheet_id, sheet = "longevity") } } # Update values to reflect the new data values(read_sheet(ss = sheet_id, sheet = "longevity")) shinyjs::hide(id = "loading") # Hide loading spinner # Generate biological age plot output$biologicalAgePlot <- renderPlot({ if (!is.null(emiglio)) { if (nrow(values() %>% filter(email == emiglio)) > 0) { values() %>% filter(email == emiglio) %>% select(c(age, age_bio, date)) %>% group_by(date) %>% mutate(age = mean(age, na.rm = TRUE), age_bio = mean(age_bio, na.rm = TRUE)) %>% rename(`Actual age` = age, `Biological age` = age_bio) %>% mutate(Date = as_date(date)) %>% pivot_longer(cols = c(1:2), names_to = "name", values_to = "value") %>% ggplot(aes(x = Date, y = value, color = name)) + geom_line(size = 1.3) + theme_bw() + scale_color_discrete(name = "") + scale_y_continuous(breaks = round(seq(min(c(values()$age, values()$age_bio)), max(c(values()$age, values()$age_bio)), by = 2), 0)) } } }) # Generate biological age table output$table <- renderTable({ if (!is.null(emiglio)) { if (nrow(values() %>% filter(email == emiglio)) > 0) { values() %>% filter(email == emiglio) %>% mutate_at(c(1, 11), round, 1) %>% select(c(15, 2:10, 1, 11)) %>% mutate(date = as.character(as.Date(date))) %>% rename( Creatinine = creat, `Lymphocyte (Lymphs)` = lymph, `CRP (C-reactive Protein)` = crp, `White Blood Cells` = wbc, `Mean Cell Volume` = mcv, `Alkaline Phosphatase` = alp, Glucose = glucose, `Red cells distribution width` = rdw, `Actual Age` = age, `Biological Age` = age_bio ) } } }) }) } shinyApp(ui = ui, server = server)