|
--- |
|
library_name: transformers |
|
tags: [] |
|
--- |
|
|
|
# ESM++ |
|
[ESM++](https://github.com/Synthyra/ESMplusplus) is a faithful implementation of [ESMC](https://www.evolutionaryscale.ai/blog/esm-cambrian) ([license](https://www.evolutionaryscale.ai/policies/cambrian-non-commercial-license-agreement)) that allows for batching and standard Huggingface compatibility without requiring the ESM Python package. |
|
The large version corresponds to the 600 million parameter version of ESMC. |
|
|
|
|
|
## Use with 🤗 transformers |
|
```python |
|
from transformers import AutoModelForMaskedLM |
|
model = AutoModelForMaskedLM.from_pretrained('Synthyra/ESMplusplus_large', trust_remote_code=True) |
|
tokenizer = model.tokenizer |
|
|
|
sequences = ['MPRTEIN', 'MSEQWENCE'] |
|
tokenized = tokenizer(sequences, padding=True, return_tensors='pt') |
|
|
|
# tokenized['labels'] = tokenized['input_ids'].clone() # correctly mask input_ids and set unmasked instances of labels to -100 for MLM training |
|
|
|
output = model(**tokenized) # get all hidden states with output_hidden_states=True |
|
print(output.logits.shape) # language modeling logits, (batch_size, seq_len, vocab_size), (2, 11, 64) |
|
print(output.last_hidden_state.shape) # last hidden state of the model, (batch_size, seq_len, hidden_size), (2, 11, 1152) |
|
print(output.loss) # language modeling loss if you passed labels |
|
#print(output.hidden_states) # all hidden states if you passed output_hidden_states=True (in tuple) |
|
``` |
|
|
|
ESM++ also supports sequence and token level classification tasks like ESM2. Simply pass the number of labels during initialization. |
|
|
|
```python |
|
from transformers import AutoModelForSequenceClassification, AutoModelForTokenClassification |
|
|
|
model = AutoModelForSequenceClassification.from_pretrained('Synthyra/ESMplusplus_large', num_labels=2, trust_remote_code=True) |
|
logits = model(**tokenized).logits |
|
print(logits.shape) # (batch_size, num_labels), (2, 2) |
|
``` |
|
|
|
ESM++ weights are fp32 by default. You can load them in fp16 or bf16 like this: |
|
```python |
|
import torch |
|
model = AutoModelForMaskedLM.from_pretrained('Synthyra/ESMplusplus_large', trust_remote_code=True, torch_dtype=torch.float16) # or torch.bfloat16 |
|
``` |
|
|
|
## Embed entire datasets with no new code |
|
To embed a list of protein sequences **fast**, just call embed_dataset. Sequences are sorted to reduce padding tokens, so the initial progress bar estimation is usually much longer than the actual time. |
|
```python |
|
embeddings = model.embed_dataset( |
|
sequences=sequences, # list of protein strings |
|
batch_size=16, # embedding batch size |
|
max_len=2048, # truncate to max_len |
|
full_embeddings=True, # return residue-wise embeddings |
|
full_precision=False, # store as float32 |
|
pooling_type='mean', # use mean pooling if protein-wise embeddings |
|
num_workers=0, # data loading num workers |
|
sql=False, # return dictionary of sequences and embeddings |
|
) |
|
|
|
_ = model.embed_dataset( |
|
sequences=sequences, # list of protein strings |
|
batch_size=16, # embedding batch size |
|
max_len=2048, # truncate to max_len |
|
full_embeddings=True, # return residue-wise embeddings |
|
full_precision=False, # store as float32 |
|
pooling_type='mean', # use mean pooling if protein-wise embeddings |
|
num_workers=0, # data loading num workers |
|
sql=True, # store sequences in local SQL database |
|
sql_db_path='embeddings.db', # path to .db file of choice |
|
) |
|
``` |
|
|
|
## Returning attention maps |
|
Usually F.scaled_dot_product_attention is used for the attention calculations, which is much faster than native PyTorch. However, it cannot return attention maps. |
|
ESM++ has the option to ```output_attentions```, which will calculate attention manually. This is much slower, so do not use unless you need the attention maps. |
|
|
|
```python |
|
output = model(**tokenized, output_attentions=True) |
|
att = output.attentions |
|
len(att) # 33, one for each layer, size (batch_size, num_heads, seq_len, seq_len) each |
|
``` |
|
|
|
## Comparison across floating-point precision and implementations |
|
We measured the difference of the last hidden states of the fp32 weights vs. fp16 or bf16. We find that the fp16 is closer to the fp32 outputs, so we recommend loading in fp16. |
|
Please note that the ESM package also loads ESMC in fp32 but casts to bf16 by default, which has its share of advantages and disadvantages in inference / training - so load whichever you like for half precision. |
|
|
|
Average MSE for FP16: 0.00000003 |
|
|
|
Average MSE for BF16: 0.00000122 |
|
|
|
We also measured the difference between the outputs of ESM++ vs. ESMC (both in bfloat16) on 1000 random sequences to ensure compliance with the ESM package. |
|
|
|
Average MSE of last hidden state: 2.46e-09 |
|
|
|
You can load the weights from the ESM package instead of transformers by replacing .from_pretrained(...) to .from_pretrained_esm('esmc_600m') |
|
|
|
## Model probes |
|
We employ linear probing techniques on various PLMs and standard datasets, similar our previous [paper](https://www.biorxiv.org/content/10.1101/2024.07.30.605924v1), to assess the intrinsic correlation between pooled hidden states and valuable properties. ESMC (and thus ESM++) perform very well. |
|
|
|
The plot below showcases performance normalized between the negative control (random vector embeddings) and the best performer. Classification task scores are averaged between MCC and F1 (or F1max for multilabel) and regression tasks are averaged between Spearman rho and R2. |
|
 |
|
|
|
## Inference speeds |
|
We look at various ESM models and their throughput on an H100. Adding efficient batching between ESMC and ESM++ significantly improves the throughput, although ESM++ is also faster than ESMC for batch size one. ESM++ small is even faster than ESM2-35M with long sequences! The most gains will be seen with PyTorch > 2.5 on linux machines. |
|
 |
|
|
|
### Citation |
|
If you use any of this implementation or work please cite it (as well as the ESMC preprint). |
|
|
|
``` |
|
@misc {ESMPlusPlus, |
|
author = { Hallee, L. and Bichara, D. and Gleghorn, J, P. }, |
|
title = { ESMPlusPlus }, |
|
year = 2024, |
|
url = { https://huggingface.co/Synthyra/ESMplusplus_small }, |
|
doi = { 10.57967/hf/3726 }, |
|
publisher = { Hugging Face } |
|
} |
|
``` |
