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Is hepatoid adenocarcinoma of the stomach a special and easily misdiagnosed or missed diagnosed subtype of gastric cancer with poor prognosis but curative for patients of pN0/1 : the experience of a single center?

The incidence of HAS among gastric cancer in our center was 0.19% (19/9915) Four out of 19 cases initially diagnosed as HAS were misdiagnosed/missed diagnosed, thus, the misdiagnosis and missed diagnosis rate was 30.8% (8/26) The incidence was 0 .19% of patients showed T stages greater than T2, 12 (70.6%) patients had positive lymph nodes in 17 available patients . The median overall survival time was 12.0 months, and the median disease-free survival time of 7.0 .

HAS remains easily being misdiagnosed/missed diagnosed based on a pathological examination, probably because the condition is rare and has various cytoplasmic types. Although the survival rate for HAS is poor, a curative effect may be achieved for N0 or N1 cases.

yes

### Human: The incidence of HAS among gastric cancer in our center was 0.19% (19/9915) Four out of 19 cases initially diagnosed as HAS were misdiagnosed/missed diagnosed, thus, the misdiagnosis and missed diagnosis rate was 30.8% (8/26) The incidence was 0 .19% of patients showed T stages greater than T2, 12 (70.6%) patients had positive lymph nodes in 17 available patients . The median overall survival time was 12.0 months, and the median disease-free survival time of 7.0 . Is hepatoid adenocarcinoma of the stomach a special and easily misdiagnosed or missed diagnosed subtype of gastric cancer with poor prognosis but curative for patients of pN0/1 : the experience of a single center?### Assistant: HAS remains easily being misdiagnosed/missed diagnosed based on a pathological examination, probably because the condition is rare and has various cytoplasmic types. Although the survival rate for HAS is poor, a curative effect may be achieved for N0 or N1 cases.

Although the survival rate for HAS is poor, a curative effect may be achieved for N0 or N1 cases .

Do risk factors before autologous stem-cell transplantation for lymphoma predict for secondary myelodysplasia and acute myelogenous leukemia?

The risk factors for treatment-related myelodysplastic syndrome (t-MDS) and acute myelogenous leukemia (AML) are similar to those that increase the risk of difficult stem-cell harvests . We reviewed our experience in 526 patients with lymphoma treated by ASCT to determine whether difficult stem cells predict an increased risk of t-mDS/AML . We found that prior exposure to radiation therapy, four or more chemotherapy regimens, and more than 5 days of apheresis needed to harvest enough stem cells were independent risk factors .

These results suggest that identifiable pretransplantation factors predict for t-MDS/AML after ASCT.

yes

### Human: The risk factors for treatment-related myelodysplastic syndrome (t-MDS) and acute myelogenous leukemia (AML) are similar to those that increase the risk of difficult stem-cell harvests . We reviewed our experience in 526 patients with lymphoma treated by ASCT to determine whether difficult stem cells predict an increased risk of t-mDS/AML . We found that prior exposure to radiation therapy, four or more chemotherapy regimens, and more than 5 days of apheresis needed to harvest enough stem cells were independent risk factors . Do risk factors before autologous stem-cell transplantation for lymphoma predict for secondary myelodysplasia and acute myelogenous leukemia?### Assistant: These results suggest that identifiable pretransplantation factors predict for t-MDS/AML after ASCT.

These results suggest that identifiable pretransplantation factors predict for t-MDS/AML after ASCT .

Does antifoam addition to shake flask cultures of recombinant Pichia pastoris increase yield?

Antifoams are often added without consideration of their effect on the yeast cells, protein product or the influence on downstream processes such as protein purification . Addition of defined concentrations of Antfoam A (Sigma) and AntifOam C (Struktol) to shake-flask cultures of Pichia pastoris increased the total amount of recombinant green fluorescent protein (GFP) in the culture medium . There was no correlation between total yield, specific yield or specific growth rate and the volumetric oxygen mass transfer coefficient (k(L)a)

We show that addition of a range of antifoaming agents to shake flask cultures of P. pastoris increases the total yield of the recombinant protein being produced. This is not only a simple method to increase the amount of protein in the culture, but our study also provides insight into how antifoams interact with microbial cell factories. Two mechanisms are apparent: one group of antifoams (Antifoam A, Antifoam C and J673A) increases the specific yield of GFP by increasing the total amount of protein produced and secreted per cell, whilst the second (P2000 or SB2121) increases the total yield by increasing the density of the culture.

yes

### Human: Antifoams are often added without consideration of their effect on the yeast cells, protein product or the influence on downstream processes such as protein purification . Addition of defined concentrations of Antfoam A (Sigma) and AntifOam C (Struktol) to shake-flask cultures of Pichia pastoris increased the total amount of recombinant green fluorescent protein (GFP) in the culture medium . There was no correlation between total yield, specific yield or specific growth rate and the volumetric oxygen mass transfer coefficient (k(L)a) Does antifoam addition to shake flask cultures of recombinant Pichia pastoris increase yield?### Assistant: We show that addition of a range of antifoaming agents to shake flask cultures of P. pastoris increases the total yield of the recombinant protein being produced. This is not only a simple method to increase the amount of protein in the culture, but our study also provides insight into how antifoams interact with microbial cell factories. Two mechanisms are apparent: one group of antifoams (Antifoam A, Antifoam C and J673A) increases the specific yield of GFP by increasing the total amount of protein produced and secreted per cell, whilst the second (P2000 or SB2121) increases the total yield by increasing the density of the culture.

Antifoaming agents to shake flask cultures of P. pastoris increases the total yield of the recombinant protein being produced . This

Does rolapitant improve quality of life of patients receiving highly or moderately emetogenic chemotherapy?

Addition of rolapitant to standard antiemetic therapy improved protection against chemotherapy-induced nausea and vomiting (CINV) in phase 3 trials of patients receiving highly emetogenic chemotherapy . Here, we assessed the impact of CINV on the daily lives of patients using the Functional Living Index-Emesis (FLIE) Endpoints included FLIE total score, nausea, vomiting domain scores, and the proportion of patients with no impact on daily life (total score >108)

Compared with control, rolapitant improved quality of life in patients receiving HEC or MEC.

yes

### Human: Addition of rolapitant to standard antiemetic therapy improved protection against chemotherapy-induced nausea and vomiting (CINV) in phase 3 trials of patients receiving highly emetogenic chemotherapy . Here, we assessed the impact of CINV on the daily lives of patients using the Functional Living Index-Emesis (FLIE) Endpoints included FLIE total score, nausea, vomiting domain scores, and the proportion of patients with no impact on daily life (total score >108) Does rolapitant improve quality of life of patients receiving highly or moderately emetogenic chemotherapy?### Assistant: Compared with control, rolapitant improved quality of life in patients receiving HEC or MEC.

Rlapitant improved quality of life in patients receiving HEC or MEC .

Does scavenger receptor class B type I regulate cellular cholesterol metabolism and cell signaling associated with breast cancer development?

Previous studies have identified cholesterol as an important regulator of breast cancer development . High-density lipoprotein (HDL) and scavenger receptor class B type I (SR-BI) have both been implicated in the regulation of cellular cholesterol homeostasis . In the study, we have examined the role of HDL and SR-BI in regulation of signaling pathways in breast cancer cell lines and in the development of tumor in a mouse xenograft model . We also show that pharmacological inhibition of the receptor can attenuate signaling and lead to decreased cellular proliferation .

These results identify SR-BI as a potential target for the treatment of breast cancer.

yes

### Human: Previous studies have identified cholesterol as an important regulator of breast cancer development . High-density lipoprotein (HDL) and scavenger receptor class B type I (SR-BI) have both been implicated in the regulation of cellular cholesterol homeostasis . In the study, we have examined the role of HDL and SR-BI in regulation of signaling pathways in breast cancer cell lines and in the development of tumor in a mouse xenograft model . We also show that pharmacological inhibition of the receptor can attenuate signaling and lead to decreased cellular proliferation . Does scavenger receptor class B type I regulate cellular cholesterol metabolism and cell signaling associated with breast cancer development?### Assistant: These results identify SR-BI as a potential target for the treatment of breast cancer.

SR-BI is a potential target for the treatment of breast cancer . SR-B is a possible target for breast cancer treatment .

Is plasma amyloid beta protein elevated in late-onset Alzheimer disease families?

We measured plasma A beta levels in first-degree relatives of patients with LOAD in a cross-sectional series and in extended families . These elevations are not due to ApoE epsilon 4 or pathogenic coding mutations in the known early-onset AD genes . These elevated levels are not caused by ApoEs epsil 4 or mutations in known AD genes, but by pathogenic mutations in these genes . We screened subjects for mutations in AD genes and determined their ApOE genotypes .

The findings provide strong evidence for the existence of novel, as yet unknown genetic factors that affect late-onset Alzheimer disease by increasing A beta.

yes

### Human: We measured plasma A beta levels in first-degree relatives of patients with LOAD in a cross-sectional series and in extended families . These elevations are not due to ApoE epsilon 4 or pathogenic coding mutations in the known early-onset AD genes . These elevated levels are not caused by ApoEs epsil 4 or mutations in known AD genes, but by pathogenic mutations in these genes . We screened subjects for mutations in AD genes and determined their ApOE genotypes . Is plasma amyloid beta protein elevated in late-onset Alzheimer disease families?### Assistant: The findings provide strong evidence for the existence of novel, as yet unknown genetic factors that affect late-onset Alzheimer disease by increasing A beta.

The findings provide strong evidence for the existence of novel, as yet unknown genetic factors that affect late-onset Alzheimer disease by increasing A

Does sulforaphane epigenetically enhance neuronal BDNF expression and TrkB signaling pathways?

Brain-derived neurotrophic factor (BDNF) is a neurotrophin that supports the survival of existing neurons and encourages the growth and differentiation of new neurons and synapses . We investigated the effect of sulforaphane, a hydrolysis product of glucoraphanin present in Brassica vegetables, on neuronal BDNF expression and its signaling pathways . We found that sulfaphane increased global acetylation of histone 3 and H3 and H4, inhibited HDAC activity, and decreased the level of HDAC2 in primary cortical neurons .

These findings suggest that sulforaphane has the potential to prevent neuronal disorders such as Alzheimer's disease by epigenetically enhancing neuronal BDNF expression and its TrkB signaling pathways.

yes

### Human: Brain-derived neurotrophic factor (BDNF) is a neurotrophin that supports the survival of existing neurons and encourages the growth and differentiation of new neurons and synapses . We investigated the effect of sulforaphane, a hydrolysis product of glucoraphanin present in Brassica vegetables, on neuronal BDNF expression and its signaling pathways . We found that sulfaphane increased global acetylation of histone 3 and H3 and H4, inhibited HDAC activity, and decreased the level of HDAC2 in primary cortical neurons . Does sulforaphane epigenetically enhance neuronal BDNF expression and TrkB signaling pathways?### Assistant: These findings suggest that sulforaphane has the potential to prevent neuronal disorders such as Alzheimer's disease by epigenetically enhancing neuronal BDNF expression and its TrkB signaling pathways.

sulforaphane has the potential to prevent neuronal disorders such as Alzheimer's disease by epigenetically enhancing neuronal BDNF expression and its Tr

Does a sonographic lesion index for Crohn 's disease help monitor changes in transmural bowel damage during therapy?

Small-intestine contrast ultrasonography (SICUS) is a valuable tool for assessing CD lesions . Little is known about the effects of anti-TNF agents on structural damage in the intestine . A sonographic quantitative index (the sonographic lesion index for CD [SLIC] was developed to quantify changes in CD lesions detected by SICUS . A clinical response was defined as steroid-free remission, with CD activity index scores less than 150 . We observed significant improvements in SLIC scores and subscores after induction and maintenance therapy .

Sonographic assessment using the quantitative index SLIC can be used to monitor changes in transmural bowel damage during anti-TNF therapy for CD.

yes

### Human: Small-intestine contrast ultrasonography (SICUS) is a valuable tool for assessing CD lesions . Little is known about the effects of anti-TNF agents on structural damage in the intestine . A sonographic quantitative index (the sonographic lesion index for CD [SLIC] was developed to quantify changes in CD lesions detected by SICUS . A clinical response was defined as steroid-free remission, with CD activity index scores less than 150 . We observed significant improvements in SLIC scores and subscores after induction and maintenance therapy . Does a sonographic lesion index for Crohn 's disease help monitor changes in transmural bowel damage during therapy?### Assistant: Sonographic assessment using the quantitative index SLIC can be used to monitor changes in transmural bowel damage during anti-TNF therapy for CD.

Sonographic assessment using the quantitative index SLIC can be used to monitor changes in transmural bowel damage during anti-TNF therapy for

Do aPOE ε variants increase risk of warfarin-related intracerebral hemorrhage?

We aimed to assess the effect of APOE ε variants on warfarin-related intracerebral hemorrhage (wICH) and evaluated their predictive power . We also tested for interaction with warfirin in causing wICH . wICH was classified as lobar or nonlobar based on the location of the hematoma . APOE variants were directly genotyped and a case-control study was carried out . No statistical interaction between warfins and APOE was found (p > 0.20)

APOE ε variants constitute strong risk factors for lobar wICH. APOE exerts its effect independently of warfarin, although power limitations render this absence of interaction preliminary. Evaluation of the predictive ability of APOE in cohort studies is warranted.

yes

### Human: We aimed to assess the effect of APOE ε variants on warfarin-related intracerebral hemorrhage (wICH) and evaluated their predictive power . We also tested for interaction with warfirin in causing wICH . wICH was classified as lobar or nonlobar based on the location of the hematoma . APOE variants were directly genotyped and a case-control study was carried out . No statistical interaction between warfins and APOE was found (p > 0.20) Do aPOE ε variants increase risk of warfarin-related intracerebral hemorrhage?### Assistant: APOE ε variants constitute strong risk factors for lobar wICH. APOE exerts its effect independently of warfarin, although power limitations render this absence of interaction preliminary. Evaluation of the predictive ability of APOE in cohort studies is warranted.

APOE exerts its effect independently of warfarin, although power limitations render this absence of interaction preliminary . Evaluation of the predictive ability

Is continuous stimulation by normal luminal bacteria essential for the development and perpetuation of colitis in Tg ( epsilon26 ) mice?

We determined whether bacterial stimulation is necessary for both induction and perpetuation of mucosal inflammation and T-cell activation in Tg(epsilon26) mice . Colitis did not develop when BM was transferred into GF recipient mice . T lymphocytes that secreted interferon gamma upon activation were present in the MLN of BM-->GF mice, albeit in lower frequency than in control BM-->SPF Tg . mice . In a reciprocal experiment, MLN cells from BM--SPF mice without colitis transferred disease to SPF recipients .

Activated T cells are present in the mucosa of BM-->GF Tg(epsilon26) mice but are incapable of inducing disease unless colonic bacteria are present. Moreover, pathogenic T cells require the continuous presence of colonic bacteria to sustain colitis.

yes

### Human: We determined whether bacterial stimulation is necessary for both induction and perpetuation of mucosal inflammation and T-cell activation in Tg(epsilon26) mice . Colitis did not develop when BM was transferred into GF recipient mice . T lymphocytes that secreted interferon gamma upon activation were present in the MLN of BM-->GF mice, albeit in lower frequency than in control BM-->SPF Tg . mice . In a reciprocal experiment, MLN cells from BM--SPF mice without colitis transferred disease to SPF recipients . Is continuous stimulation by normal luminal bacteria essential for the development and perpetuation of colitis in Tg ( epsilon26 ) mice?### Assistant: Activated T cells are present in the mucosa of BM-->GF Tg(epsilon26) mice but are incapable of inducing disease unless colonic bacteria are present. Moreover, pathogenic T cells require the continuous presence of colonic bacteria to sustain colitis.

Pathogenic T cells require the continuous presence of colonic bacteria to sustain colitis .

Do [ Transthoracic and transesophageal echo Doppler in the detection of mitral prosthesis dysfunction due to insufficiency ]?

Fourty-two patients were studied . The objective of this work was to compare Doppler parameters related to transvalvular peak-pressure gradient in patients with normally functioning and patients with malfunctioning mitral prosthetic valve, secondary to insufficiency . All of the patients were evaluated though transthoracic (TTE) and transesophageal (TEE) Dopplers . Diastolic peak velocity prosthetic flow (DVP), protodiastolic gradient (PDG), mean gradient (MG) and prosthetic area (PA) by pressure halftime were measured .

In patients with high PDG (greater than 20 mmHg) and normal prosthetic mitral area assessed by TTE could be suspicious of malfunctioning prosthesis secondary to severe insufficiency. Due to the higher sensitivity and specificity in the diagnosis of malfunctioning mitral prosthesis and the ability to determine the magnitude and severity of prosthetic regurgitation assessment should be completed with TTE.

yes

### Human: Fourty-two patients were studied . The objective of this work was to compare Doppler parameters related to transvalvular peak-pressure gradient in patients with normally functioning and patients with malfunctioning mitral prosthetic valve, secondary to insufficiency . All of the patients were evaluated though transthoracic (TTE) and transesophageal (TEE) Dopplers . Diastolic peak velocity prosthetic flow (DVP), protodiastolic gradient (PDG), mean gradient (MG) and prosthetic area (PA) by pressure halftime were measured . Do [ Transthoracic and transesophageal echo Doppler in the detection of mitral prosthesis dysfunction due to insufficiency ]?### Assistant: In patients with high PDG (greater than 20 mmHg) and normal prosthetic mitral area assessed by TTE could be suspicious of malfunctioning prosthesis secondary to severe insufficiency. Due to the higher sensitivity and specificity in the diagnosis of malfunctioning mitral prosthesis and the ability to determine the magnitude and severity of prosthetic regurgitation assessment should be completed with TTE.

In patients with high PDG (greater than 20 mmHg) and normal prosthetic mitral area assessed by TTE could

Is lomefloxacin an effective treatment of experimental bacterial keratitis?

Lomefloxacin was compared with ciprofloxain in different rabbit keratitis models . The data was analyzed statistically comparing the decrease in the number of recovered viable bacteria . For gram-positive bacteria, lomefloxinacin and ciprafloxacoin were equally effective . For Gram-negative bacteria, the effective lomefoxacins were less effective than ciprroflaxacin under experimental conditions (p < 0.05) for gram-negative bacterial isolates .

Our data, using multiple bacterial keratitis models, suggest that lomefloxacin is promising for therapy of bacterial keratitis. Further clinical studies are needed to expand its use for keratitis therapy.

yes

### Human: Lomefloxacin was compared with ciprofloxain in different rabbit keratitis models . The data was analyzed statistically comparing the decrease in the number of recovered viable bacteria . For gram-positive bacteria, lomefloxinacin and ciprafloxacoin were equally effective . For Gram-negative bacteria, the effective lomefoxacins were less effective than ciprroflaxacin under experimental conditions (p < 0.05) for gram-negative bacterial isolates . Is lomefloxacin an effective treatment of experimental bacterial keratitis?### Assistant: Our data, using multiple bacterial keratitis models, suggest that lomefloxacin is promising for therapy of bacterial keratitis. Further clinical studies are needed to expand its use for keratitis therapy.

Lomefloxacin is promising for therapy of bacterial keratitis . Further clinical studies needed to expand its use for kerat

Are expression of integrin α3β1 and cyclooxygenase-2 ( COX2 ) positively correlated in human breast cancer?

Integrin α3β1 promotes expression of cyclooxygenase-2 (COX2/PTGS2), a known driver of breast cancer progression . However, the clinical significance of this regulation was unknown . The objective of the current study was to assess the clinical relevance of the relationship between integrin and COX2 by testing for their correlated expression among various forms of human breast cancer . In both sample sets, this correlation was independent of hormone receptor status, histological grade, or disease stage .

COX2 and α3 are correlated in IDC independently of hormone receptor status or other clinicopathologic features, supporting the hypothesis that integrin α3β1 is a determinant of COX2 expression in human breast cancer. These results support the clinical relevance of α3β1-dependent COX2 gene expression that we reported previously in breast cancer cells. The findings also suggest that COX2-positive breast carcinomas of various subtypes might be vulnerable to therapeutic strategies that target α3β1, and that α3 expression might serve as an independent prognostic biomarker.

yes

### Human: Integrin α3β1 promotes expression of cyclooxygenase-2 (COX2/PTGS2), a known driver of breast cancer progression . However, the clinical significance of this regulation was unknown . The objective of the current study was to assess the clinical relevance of the relationship between integrin and COX2 by testing for their correlated expression among various forms of human breast cancer . In both sample sets, this correlation was independent of hormone receptor status, histological grade, or disease stage . Are expression of integrin α3β1 and cyclooxygenase-2 ( COX2 ) positively correlated in human breast cancer?### Assistant: COX2 and α3 are correlated in IDC independently of hormone receptor status or other clinicopathologic features, supporting the hypothesis that integrin α3β1 is a determinant of COX2 expression in human breast cancer. These results support the clinical relevance of α3β1-dependent COX2 gene expression that we reported previously in breast cancer cells. The findings also suggest that COX2-positive breast carcinomas of various subtypes might be vulnerable to therapeutic strategies that target α3β1, and that α3 expression might serve as an independent prognostic biomarker.

COX2 and α3 are correlated in IDC independently of hormone receptor status or other clinicopathologic features . This supports the hypothesis

Is ubp43 gene expression required for normal Isg15 expression and fetal development?

Isg15 can be severed from targeted proteins by a specific protease called Ubp43 (Usp18) Mice lacking Ubp 43 form increased conjugated Isg 15 in response to interferon . The IsG15 system has not been examined in chorioallantoic placenta (CP) or mesometrial (MM) components of implantation sites beyond 9.5 days post coitum . It was hypothesized that deletion of Ubp42 would cause disregulation of Isg

It is concluded that Isg15 and its conjugates are present in implantation sites during mid to late gestation and that deletion of Ubp43 causes an increase in free and conjugated Isg15 at the feto-maternal interface. Also, under mixed genetic background, deletion of Ubp43 results in fetal death.

yes

### Human: Isg15 can be severed from targeted proteins by a specific protease called Ubp43 (Usp18) Mice lacking Ubp 43 form increased conjugated Isg 15 in response to interferon . The IsG15 system has not been examined in chorioallantoic placenta (CP) or mesometrial (MM) components of implantation sites beyond 9.5 days post coitum . It was hypothesized that deletion of Ubp42 would cause disregulation of Isg Is ubp43 gene expression required for normal Isg15 expression and fetal development?### Assistant: It is concluded that Isg15 and its conjugates are present in implantation sites during mid to late gestation and that deletion of Ubp43 causes an increase in free and conjugated Isg15 at the feto-maternal interface. Also, under mixed genetic background, deletion of Ubp43 results in fetal death.

Isg15 and its conjugates are present in implantation sites during mid to late gestation . Dehumanisation of Ubp43

Does exposure to cigarette smoke impair alveolar macrophage functions during halothane and isoflurane anesthesia in rats?

Smoke exposure impairs antimicrobial and proinflammatory responses in alveolar macrophages during halothane and isoflurane anesthesia with mechanical ventilation . Responses observed in smoke-exposed rats were almost twice as great as those observed in the control rats . Gene expression and production of all proinflammatory cytokines except IL-6 increased 2-20-fold during anesthesia . The increases in IL-1beta, interferon-gamma, and tumor necrosis factor-alpha in control rats were 1.5-8 times greater .

Antimicrobial and proinflammatory responses of alveolar macrophages during anesthesia were markedly suppressed by smoke exposure. Our data suggest that smoke exposure reduces the efficacy of immune defenses during anesthesia.

yes

### Human: Smoke exposure impairs antimicrobial and proinflammatory responses in alveolar macrophages during halothane and isoflurane anesthesia with mechanical ventilation . Responses observed in smoke-exposed rats were almost twice as great as those observed in the control rats . Gene expression and production of all proinflammatory cytokines except IL-6 increased 2-20-fold during anesthesia . The increases in IL-1beta, interferon-gamma, and tumor necrosis factor-alpha in control rats were 1.5-8 times greater . Does exposure to cigarette smoke impair alveolar macrophage functions during halothane and isoflurane anesthesia in rats?### Assistant: Antimicrobial and proinflammatory responses of alveolar macrophages during anesthesia were markedly suppressed by smoke exposure. Our data suggest that smoke exposure reduces the efficacy of immune defenses during anesthesia.

Smoke exposure reduces the efficacy of immune defenses during anesthesia .

Does α-Tocopherol ameliorate nickel induced testicular oxidative and nitrosative stress in albino rats?

In this study, we have evaluated the beneficial effects of α-tocopherol against nickel sulfate exposed testicular dysfunction . We also evaluated sperm count, motility and histopathology of testes . Nickel sulfate induced testicular oxidative and nitrosative stress in Wister strain male albino rats . We studied the effect of the supplementation of the antioxidant α-tocopherol (10 mg/100 g body weight, i.m.m.) on the effect .

Nickel sulfate treatment causes testicular oxidative and nitrosative stress in albino rats, but simultaneous supplementation of α-tocopherol was found to be beneficial in combating against such stresses.

yes

### Human: In this study, we have evaluated the beneficial effects of α-tocopherol against nickel sulfate exposed testicular dysfunction . We also evaluated sperm count, motility and histopathology of testes . Nickel sulfate induced testicular oxidative and nitrosative stress in Wister strain male albino rats . We studied the effect of the supplementation of the antioxidant α-tocopherol (10 mg/100 g body weight, i.m.m.) on the effect . Does α-Tocopherol ameliorate nickel induced testicular oxidative and nitrosative stress in albino rats?### Assistant: Nickel sulfate treatment causes testicular oxidative and nitrosative stress in albino rats, but simultaneous supplementation of α-tocopherol was found to be beneficial in combating against such stresses.

Nickel sulfate treatment causes testicular oxidative and nitrosative stress in albino rats . But simultaneous supplementation of α-tocophe

Is measurement of human intercellular adhesion molecule 1 in the blood useful for predicting the occurrence of hepatocellular carcinomas from chronic hepatitis C and liver cirrhosis?

We measured the concentrations of serum intercellular adhesion molecule 1 (sICAM-1) in patients with C-viral chronic liver diseases . We studied 74 patients with CH, 18 with LC, and 28 patients with HCC who visited our institute from 1993 through 1996 . The concentration of ICAM- 1 was significantly higher in patients who had low platelet counts . ICAM1 in the liver was localized to the endoplasmic reticulum or the membrane of cancer cells . The cumulative rate of occurrence of HCC from CH or LC was significantly .

Evaluation of the sICAM-1 concentration is useful for prediction of the occurrence of HCC in patients with C-viral CH or LC.

yes

### Human: We measured the concentrations of serum intercellular adhesion molecule 1 (sICAM-1) in patients with C-viral chronic liver diseases . We studied 74 patients with CH, 18 with LC, and 28 patients with HCC who visited our institute from 1993 through 1996 . The concentration of ICAM- 1 was significantly higher in patients who had low platelet counts . ICAM1 in the liver was localized to the endoplasmic reticulum or the membrane of cancer cells . The cumulative rate of occurrence of HCC from CH or LC was significantly . Is measurement of human intercellular adhesion molecule 1 in the blood useful for predicting the occurrence of hepatocellular carcinomas from chronic hepatitis C and liver cirrhosis?### Assistant: Evaluation of the sICAM-1 concentration is useful for prediction of the occurrence of HCC in patients with C-viral CH or LC.

sICAM-1 concentration is useful for prediction of the occurrence of HCC in patients with C-viral CH or LC .

Do cHA2DS2-VASc and R2CHA2DS2-VASc scores have predictive value in patients with acute coronary syndromes?

Late-onset systemic lupus erythematosus (SLE) diagnosed in patients over the age of 50 is estimated at 10% to 20% . SLE in elderly patients has specific features with misleading signs and symptoms, but its clinical course seems milder compared with that in younger patients . The most common SLE features were arthritis (50%), rash (40%), nephropathy (40%) photosensitivity (30%), photosensitivity) and mouth ulcerations . Kidney involvement was present in all men and in 25% of women .

The clinical picture of late-onset SLE differs from that of early-onset SLE. Arthritis, leukopenia, and thrombotic complications are frequent, while skin manifestations, photosensitivity, nephropathy, vasculitis, and central nervous system involvement are less common in late-onset SLE. The diagnosis of late-onset SLE is often delayed, and treatment is determined by the presence of concomitant diseases.

yes

### Human: Late-onset systemic lupus erythematosus (SLE) diagnosed in patients over the age of 50 is estimated at 10% to 20% . SLE in elderly patients has specific features with misleading signs and symptoms, but its clinical course seems milder compared with that in younger patients . The most common SLE features were arthritis (50%), rash (40%), nephropathy (40%) photosensitivity (30%), photosensitivity) and mouth ulcerations . Kidney involvement was present in all men and in 25% of women . Do cHA2DS2-VASc and R2CHA2DS2-VASc scores have predictive value in patients with acute coronary syndromes?### Assistant: The clinical picture of late-onset SLE differs from that of early-onset SLE. Arthritis, leukopenia, and thrombotic complications are frequent, while skin manifestations, photosensitivity, nephropathy, vasculitis, and central nervous system involvement are less common in late-onset SLE. The diagnosis of late-onset SLE is often delayed, and treatment is determined by the presence of concomitant diseases.

Arthritis, leukopenia, and thrombotic complications are frequent in late-onset SLE . Skin manifestations,

Does hypoxia augment oscillatory blood flow in brachial artery during leg cycling?

Eight males participated in the study . Subjects inhaled a gas mixture while inhaling a normoxic gas mixture . Brachial artery blood velocity and diameter were simultaneously recorded during exercise using Doppler ultrasonography . The extent of changes in antegrade and retrograde blood flows during submaximal exercise in hypoxia was greater than that in normoxia . We hypothesized that oscillatory antegrade . blood flows to the nonworking limb would increase during incremental cycle exercise under hypoxic condition .

These results indicate that hypoxia has a significant effect on oscillatory antegrade/retrograde blood flow patterns in nonworking limb during cycling exercise.

yes

### Human: Eight males participated in the study . Subjects inhaled a gas mixture while inhaling a normoxic gas mixture . Brachial artery blood velocity and diameter were simultaneously recorded during exercise using Doppler ultrasonography . The extent of changes in antegrade and retrograde blood flows during submaximal exercise in hypoxia was greater than that in normoxia . We hypothesized that oscillatory antegrade . blood flows to the nonworking limb would increase during incremental cycle exercise under hypoxic condition . Does hypoxia augment oscillatory blood flow in brachial artery during leg cycling?### Assistant: These results indicate that hypoxia has a significant effect on oscillatory antegrade/retrograde blood flow patterns in nonworking limb during cycling exercise.

Hypoxia has a significant effect on oscillatory antegrade/retrograde blood flow patterns in nonworking limb during cycling exercise .

Is cRP reduction following gastric bypass surgery most pronounced in insulin-sensitive subjects?

In morbidly obese patients, gastric bypass surgery causes marked changes in body weight and improves metabolism . Study of 66 obese subjects with mean age 39 y and mean body mass index 45 kg/m2 were studied prior to and 6 and 12 months following Roux-en-Y surgery . Surgery reduced BMI from 45 to 31 kg/ m2 and lowered CRP, SAA and IL-6 levels by 82, 57 and 50%, respectively, at 12 months . Reductions in SAA, IL-4 and IL

In morbidly obese subjects, gastric bypass surgery lowers energy intake, reduces inflammatory markers and improves insulin sensitivity. Despite a marked reduction in body weight, only a small effect on CRP levels was seen in insulin-resistant patients, indicating that flexibility of circulating CRP levels is primarily dependent upon insulin sensitivity rather than energy supply.

yes

### Human: In morbidly obese patients, gastric bypass surgery causes marked changes in body weight and improves metabolism . Study of 66 obese subjects with mean age 39 y and mean body mass index 45 kg/m2 were studied prior to and 6 and 12 months following Roux-en-Y surgery . Surgery reduced BMI from 45 to 31 kg/ m2 and lowered CRP, SAA and IL-6 levels by 82, 57 and 50%, respectively, at 12 months . Reductions in SAA, IL-4 and IL Is cRP reduction following gastric bypass surgery most pronounced in insulin-sensitive subjects?### Assistant: In morbidly obese subjects, gastric bypass surgery lowers energy intake, reduces inflammatory markers and improves insulin sensitivity. Despite a marked reduction in body weight, only a small effect on CRP levels was seen in insulin-resistant patients, indicating that flexibility of circulating CRP levels is primarily dependent upon insulin sensitivity rather than energy supply.

In morbidly obese subjects, gastric bypass surgery lowers energy intake, reduces inflammatory markers and improves insulin sensitivity . Despite a marked reduction in

Does fusion cell vaccination of patients with metastatic breast and renal cancer induce immunological and clinical responses?

Dendritic cells (DCs) are potent antigen-presenting cells that are uniquely capable of inducing tumor-specific immune responses . We conducted a Phase I trial in which patients with metastatic breast and renal cancer were treated with a vaccine prepared by fusing autologous tumor and DCs . Fusion cells coexpressed tumors and DC antigens and stimulated allogeneic T-cell proliferation . There was no significant treatment-related toxicity and no clinical evidence of autoimmunity .

Our findings demonstrate that fusion cell vaccination of patients with metastatic breast and renal cancer is a feasible, nontoxic approach associated with the induction of immunological and clinical antitumor responses.

yes

### Human: Dendritic cells (DCs) are potent antigen-presenting cells that are uniquely capable of inducing tumor-specific immune responses . We conducted a Phase I trial in which patients with metastatic breast and renal cancer were treated with a vaccine prepared by fusing autologous tumor and DCs . Fusion cells coexpressed tumors and DC antigens and stimulated allogeneic T-cell proliferation . There was no significant treatment-related toxicity and no clinical evidence of autoimmunity . Does fusion cell vaccination of patients with metastatic breast and renal cancer induce immunological and clinical responses?### Assistant: Our findings demonstrate that fusion cell vaccination of patients with metastatic breast and renal cancer is a feasible, nontoxic approach associated with the induction of immunological and clinical antitumor responses.

fusion cell vaccination of patients with metastatic breast and renal cancer is a feasible, nontoxic approach . The vaccine is a nontoxic,

Is the PTPN22 gene associated with idiopathic inflammatory myopathy?

The aim of this study was to determine whether a single-nucleotide polymorphism (SNP; 1858CT, R620W) in the protein tyrosine phosphatase N22 (PTPN22) gene confers susceptibility to idiopathic inflammatory myopathy (IIM) in patients with IIM . The PTPN 22 R620w minor allele frequency was increased in IIM patients (50 of 398, 12.6%) compared with controls (75 of 910)

The PTPN22 R620W minor allele is associated with susceptibility to IIM in SA patients, independent of the 8.1 AH. Muscle Nerve, 2016.

yes

### Human: The aim of this study was to determine whether a single-nucleotide polymorphism (SNP; 1858CT, R620W) in the protein tyrosine phosphatase N22 (PTPN22) gene confers susceptibility to idiopathic inflammatory myopathy (IIM) in patients with IIM . The PTPN 22 R620w minor allele frequency was increased in IIM patients (50 of 398, 12.6%) compared with controls (75 of 910) Is the PTPN22 gene associated with idiopathic inflammatory myopathy?### Assistant: The PTPN22 R620W minor allele is associated with susceptibility to IIM in SA patients, independent of the 8.1 AH. Muscle Nerve, 2016.

The PTPN22 R620W minor allele is associated with susceptibility to IIM in SA patients, independent of the 8.1

Is severe hypoglycemia a serious complication and becoming an economic burden in diabetes?

The prevalence of hypoglycemia is increasing due to the growing incidence of diabetes and the latest strict guidelines for glycated hemoglobin (HbA1c) levels under 7% . This study examined the clinical characteristics, causal factors, and medical costs of severely hypoglycemic patients in an emergency room (ER) of Uijeongbu St. Mary's Hospital . The study consisted of a retrospective analysis of the characteristics, risk factors and medical . costs of 320 severely . patients with diabetes . from January 1, 2006 to December 31, 2009 .

Severe hypoglycemia is frequent in older diabetic patients, subjects with low HbA1c levels, and nephropathic patients. Therefore, personalized attention is warranted, especially in long-term diabetics with multiple comorbidities who may not have been properly educated or may need re-education for hypoglycemia.

yes

### Human: The prevalence of hypoglycemia is increasing due to the growing incidence of diabetes and the latest strict guidelines for glycated hemoglobin (HbA1c) levels under 7% . This study examined the clinical characteristics, causal factors, and medical costs of severely hypoglycemic patients in an emergency room (ER) of Uijeongbu St. Mary's Hospital . The study consisted of a retrospective analysis of the characteristics, risk factors and medical . costs of 320 severely . patients with diabetes . from January 1, 2006 to December 31, 2009 . Is severe hypoglycemia a serious complication and becoming an economic burden in diabetes?### Assistant: Severe hypoglycemia is frequent in older diabetic patients, subjects with low HbA1c levels, and nephropathic patients. Therefore, personalized attention is warranted, especially in long-term diabetics with multiple comorbidities who may not have been properly educated or may need re-education for hypoglycemia.

Severe hypoglycemia is frequent in older diabetic patients, subjects with low HbA1c levels, and nephrop

Does novel electrical stimulation set the cultured myoblast contractile function to 'on '?

L6 rat myoblasts were electrically stimulated by newly developed methods on culture days 6, 8, 10 and 12 . Electrical stimulation accelerated the appearance of myotubes, and subsequently produced spontaneously contracting muscle fibers . Measurement of membrane potential showed that the contracting cell had functional ion channels and gap junctional intercellular communication . An enhanced expression of MyoD family and M-cadherin was also observed . Expression of connexin 43 was increased and maintained at a high level .

This is the first demonstration of in vitro induction of myoblasts in spontaneously contractile muscle fibers by intermittent stimulation. This novel method for induction of myoblast differentiation represents an important advance in cell therapy.

yes

### Human: L6 rat myoblasts were electrically stimulated by newly developed methods on culture days 6, 8, 10 and 12 . Electrical stimulation accelerated the appearance of myotubes, and subsequently produced spontaneously contracting muscle fibers . Measurement of membrane potential showed that the contracting cell had functional ion channels and gap junctional intercellular communication . An enhanced expression of MyoD family and M-cadherin was also observed . Expression of connexin 43 was increased and maintained at a high level . Does novel electrical stimulation set the cultured myoblast contractile function to 'on '?### Assistant: This is the first demonstration of in vitro induction of myoblasts in spontaneously contractile muscle fibers by intermittent stimulation. This novel method for induction of myoblast differentiation represents an important advance in cell therapy.

This is the first demonstration of in vitro induction of myoblasts in spontaneously contractile muscle fibers by intermittent stimulation .

Does the differential expression of mgl mRNA by Porphyromonas gingivalis affect the production of methyl mercaptan?

Porphyromonas gingivalis (Pg), a major periodontopathogenic bacterium, was isolated from patients with halitosis by the amplification of 16S rRNA . The ability of isolated Pg to produce methyl mercaptan (CH3 SH) was determined to clarify the relationship between halitis and Pg infection . The production of CH3 SH and the expression of mgl mRNA were also determined to assess the effects of oriental medicine . The expression of l-methionine-a-deamino-g-mercaptomethane-lyase was strongly inhibited by magnoliaceae .

The production of CH3 SH was correlated with the expression of mgl. Furthermore, the oriental medicine, magnoliaceae, may represent a potential treatment for halitosis.

yes

### Human: Porphyromonas gingivalis (Pg), a major periodontopathogenic bacterium, was isolated from patients with halitosis by the amplification of 16S rRNA . The ability of isolated Pg to produce methyl mercaptan (CH3 SH) was determined to clarify the relationship between halitis and Pg infection . The production of CH3 SH and the expression of mgl mRNA were also determined to assess the effects of oriental medicine . The expression of l-methionine-a-deamino-g-mercaptomethane-lyase was strongly inhibited by magnoliaceae . Does the differential expression of mgl mRNA by Porphyromonas gingivalis affect the production of methyl mercaptan?### Assistant: The production of CH3 SH was correlated with the expression of mgl. Furthermore, the oriental medicine, magnoliaceae, may represent a potential treatment for halitosis.

The production of CH3 SH was correlated with the expression of mgl. Furthermore, the oriental medicine, magnoliaceae, may

Is presence of tumor cells in bone marrow but not in blood associated with adverse prognosis in patients with Ewing 's tumor . Société Française d'Oncologie Pédiatrique?

Gene fusions that result from chromosome translocations observed in Ewing's tumor provide tumor-specific markers . These markers were used to evaluate, at diagnosis, a series of 67 ET patients . 16 of 62 (26%) patients had circulating tumor cells . This was not correlated with any clinical parameters . In contrast, Ewing’s cells were detected by RT-PCR in BM in 14 of 43 (33%) patients and were associated with the presence of clinically detectable metastases and a statistically significant unfavorable outcome in univariate analysis .

These results suggested that the monitoring of BM but not of PB by RT-PCR might constitute an important criterion for the staging, at diagnosis, of patients with ET. Further studies should appreciate the relationship or independence of this marker toward other classical prognostic factors in ET, particularly to the presence of clinically detectable metastases.

yes

### Human: Gene fusions that result from chromosome translocations observed in Ewing's tumor provide tumor-specific markers . These markers were used to evaluate, at diagnosis, a series of 67 ET patients . 16 of 62 (26%) patients had circulating tumor cells . This was not correlated with any clinical parameters . In contrast, Ewing’s cells were detected by RT-PCR in BM in 14 of 43 (33%) patients and were associated with the presence of clinically detectable metastases and a statistically significant unfavorable outcome in univariate analysis . Is presence of tumor cells in bone marrow but not in blood associated with adverse prognosis in patients with Ewing 's tumor . Société Française d'Oncologie Pédiatrique?### Assistant: These results suggested that the monitoring of BM but not of PB by RT-PCR might constitute an important criterion for the staging, at diagnosis, of patients with ET. Further studies should appreciate the relationship or independence of this marker toward other classical prognostic factors in ET, particularly to the presence of clinically detectable metastases.

Monitoring of BM but not PB by RT-PCR might constitute an important criterion for the staging, at diagnosis, of patients with ET

Is imiquimod 5 % cream an acceptable treatment option for external anogenital warts in uncircumcised males?

An open-label study in six UK medical centres with 35 uncircumcised males treated with imiquimod 5% cream three times per week for up to 16 weeks . Forty per cent of patients had complete clearance of anogenital warts within 16 weeks. Three times weekly application of erythema was found to be safe, with ery the most commonly reported local skin reaction. 40 per cent had complete clearances of warts in 16 weeks, with the most common skin reaction being the most frequently reported local reaction to the treatment .

Imiquimod cream at a dosing regimen of three times per week, is effective and has an acceptable safety profile in the treatment of prepuce associated warts and other external anogenital warts in uncircumcised males.

yes

### Human: An open-label study in six UK medical centres with 35 uncircumcised males treated with imiquimod 5% cream three times per week for up to 16 weeks . Forty per cent of patients had complete clearance of anogenital warts within 16 weeks. Three times weekly application of erythema was found to be safe, with ery the most commonly reported local skin reaction. 40 per cent had complete clearances of warts in 16 weeks, with the most common skin reaction being the most frequently reported local reaction to the treatment . Is imiquimod 5 % cream an acceptable treatment option for external anogenital warts in uncircumcised males?### Assistant: Imiquimod cream at a dosing regimen of three times per week, is effective and has an acceptable safety profile in the treatment of prepuce associated warts and other external anogenital warts in uncircumcised males.

Imiquimod cream at a dosing regimen of three times per week, is effective and has an acceptable safety profile in the treatment

Does cyclophosphamide facilitate antitumor efficacy against subcutaneous tumors following intravenous delivery of reovirus?

The purpose of the study was to investigate whether it is possible to achieve truly systemic delivery of oncolytic reovirus, in immunocompetent hosts, using cyclophosphamide to overcome some of the barriers to effective intratumoral delivery and replication of i.v. injected virus . The study was conducted in mice bearing established s.c. B16 tumors . Intratumoral viral replication, tumor size, survival, and survival were measured along with levels of neutralizing antibody (NAb) in the blood .

NAb to oncolytic viruses play a dual role in the context of systemic viral delivery; on one hand, they hinder repeated administration of virus but on the other, they provide an important safety mechanism by which virus released from vigorous intratumoral replication is neutralized before it can disseminate and cause toxicity. These data support the use of cyclophosphamide to modulate, but not ablate, patient NAb, in development of carefully controlled clinical trials of the systemic administration of oncolytic viruses.

yes

### Human: The purpose of the study was to investigate whether it is possible to achieve truly systemic delivery of oncolytic reovirus, in immunocompetent hosts, using cyclophosphamide to overcome some of the barriers to effective intratumoral delivery and replication of i.v. injected virus . The study was conducted in mice bearing established s.c. B16 tumors . Intratumoral viral replication, tumor size, survival, and survival were measured along with levels of neutralizing antibody (NAb) in the blood . Does cyclophosphamide facilitate antitumor efficacy against subcutaneous tumors following intravenous delivery of reovirus?### Assistant: NAb to oncolytic viruses play a dual role in the context of systemic viral delivery; on one hand, they hinder repeated administration of virus but on the other, they provide an important safety mechanism by which virus released from vigorous intratumoral replication is neutralized before it can disseminate and cause toxicity. These data support the use of cyclophosphamide to modulate, but not ablate, patient NAb, in development of carefully controlled clinical trials of the systemic administration of oncolytic viruses.

NAb to oncolytic viruses play a dual role in the context of systemic viral delivery . They hinder repeated administration of virus but

Are wnt modulators in the aqueous humor associated with outer retinal damage severity in patients with neovascular age-related macular degeneration?

Wnt modulators Wnt inhibitory factor 1 (WIF-1) and Dickkopf 3 (DKK-3) in the aqueous humor with neovascular age-related macular degeneration (nAMD) were studied . Western blotting showed increased WIF1 (pre) and DKK3 in 12 nAMD patients compared with 12 controls . High WIF 1 levels were associated with increased disruption in the photoreceptor junction's inner and outer segments (IS/OS) (pre and post) and high WIF- 1 (post) levels .

We report, for the first time, the possibility of phenotypic, anatomic, and ocular proteomic correlations, demonstrating correlated WIF-1 and DKK-3 upregulation in nAMD patients' aqueous humor. Secreted WIF-1, reflecting the degree of retinal structure damage, may be a new biomarker for the retina's healthy and disease states.

yes

### Human: Wnt modulators Wnt inhibitory factor 1 (WIF-1) and Dickkopf 3 (DKK-3) in the aqueous humor with neovascular age-related macular degeneration (nAMD) were studied . Western blotting showed increased WIF1 (pre) and DKK3 in 12 nAMD patients compared with 12 controls . High WIF 1 levels were associated with increased disruption in the photoreceptor junction's inner and outer segments (IS/OS) (pre and post) and high WIF- 1 (post) levels . Are wnt modulators in the aqueous humor associated with outer retinal damage severity in patients with neovascular age-related macular degeneration?### Assistant: We report, for the first time, the possibility of phenotypic, anatomic, and ocular proteomic correlations, demonstrating correlated WIF-1 and DKK-3 upregulation in nAMD patients' aqueous humor. Secreted WIF-1, reflecting the degree of retinal structure damage, may be a new biomarker for the retina's healthy and disease states.

Secreted WIF-1 reflects the degree of retinal structure damage, may be a new biomarker for the retina's healthy and

Are prostate-specific antigen doubling times similar in patients with recurrence after radical prostatectomy or radiotherapy : a novel analysis?

Some investigators have analyzed the rate of growth of prostate cancer recurred after definitive radiotherapy or radical prostatectomy using serum prostate-specific antigen (PSA) doubling times (DT) We examined all PSA values in recurrent patients to determine the pattern and rate of increase in PSA after radiation therapy and radical prostateCTomy . Rate of PSA DT was not assumed to be constant over time; it was instead allowed to vary . We use a piecewise linear random-coefficient model in time for log, which allowed different mean models for both treatments .

A piecewise linear random-coefficients model enables interval analysis of PSA DT. While the PSA DT after radiation therapy and radical prostatectomy are different in the first 2 years, the rate of increase in PSA appears to be similar in the two groups after year 2, which suggests the rate of growth of cancers that recur after radiation therapy and radical prostatectomy is similar.

yes

### Human: Some investigators have analyzed the rate of growth of prostate cancer recurred after definitive radiotherapy or radical prostatectomy using serum prostate-specific antigen (PSA) doubling times (DT) We examined all PSA values in recurrent patients to determine the pattern and rate of increase in PSA after radiation therapy and radical prostateCTomy . Rate of PSA DT was not assumed to be constant over time; it was instead allowed to vary . We use a piecewise linear random-coefficient model in time for log, which allowed different mean models for both treatments . Are prostate-specific antigen doubling times similar in patients with recurrence after radical prostatectomy or radiotherapy : a novel analysis?### Assistant: A piecewise linear random-coefficients model enables interval analysis of PSA DT. While the PSA DT after radiation therapy and radical prostatectomy are different in the first 2 years, the rate of increase in PSA appears to be similar in the two groups after year 2, which suggests the rate of growth of cancers that recur after radiation therapy and radical prostatectomy is similar.

PSA DT after radiation therapy and radical prostatectomy are different in the first 2 years . Rate of increase in PSA appears to

Is concussion history a predictor of computerised neurocognitive performance?

Long term effects of self reported concussion on neurocognitive functioning have been found to be variable . Multivariate analysis of variance was used to evaluate differences between the concussion history groups on the two computer based concussion assessment programs . No significant differences between groups on either the ImPACT test and the Headminder CRI test . No differences between those with and without a history of concussion on the CRI (Lambda = 0.963, F((15, 627.05) = 0 .57, p .898)

The results suggest that either long term cognitive decrements may not be associated with a history of concussion or the decrements may be subtle and undetectable by these computer programs.

no

### Human: Long term effects of self reported concussion on neurocognitive functioning have been found to be variable . Multivariate analysis of variance was used to evaluate differences between the concussion history groups on the two computer based concussion assessment programs . No significant differences between groups on either the ImPACT test and the Headminder CRI test . No differences between those with and without a history of concussion on the CRI (Lambda = 0.963, F((15, 627.05) = 0 .57, p .898) Is concussion history a predictor of computerised neurocognitive performance?### Assistant: The results suggest that either long term cognitive decrements may not be associated with a history of concussion or the decrements may be subtle and undetectable by these computer programs.

The results suggest that long term cognitive decrements may not be associated with a history of concussion . The decrements are subtle and undetect

Does expression profiling reveal MSX1 and EphB2 expression correlates with the invasion capacity of Wilms tumors?

Wilms tumor is the most common pediatric renal malignancy . The parameters that are important to its invasion capacity are poorly understood . The aim of this study was to identify new proteins associated with the invasion capacity of Wilms tumors . Gene expression profiles for selected genes were determined by Affymetrix Genechip® Human Genome Ul33A microarray analysis . The differential expression for Bcl2A1, EphB2, MSX1, and RIN1 was confirmed by quantitative RT-PCR analysis .

Our results indicate that MSX1 may be associated with the invasion capacity of Wilms tumors. RIN1 is a downstream effector of RAS and Bcl2A1 functions as an anti-apoptotic protein. EphB2 is an ephrin receptor and is up-regulated in invasive tumors but its role needs to be confirmed in further cases of Wilms tumors.

yes

### Human: Wilms tumor is the most common pediatric renal malignancy . The parameters that are important to its invasion capacity are poorly understood . The aim of this study was to identify new proteins associated with the invasion capacity of Wilms tumors . Gene expression profiles for selected genes were determined by Affymetrix Genechip® Human Genome Ul33A microarray analysis . The differential expression for Bcl2A1, EphB2, MSX1, and RIN1 was confirmed by quantitative RT-PCR analysis . Does expression profiling reveal MSX1 and EphB2 expression correlates with the invasion capacity of Wilms tumors?### Assistant: Our results indicate that MSX1 may be associated with the invasion capacity of Wilms tumors. RIN1 is a downstream effector of RAS and Bcl2A1 functions as an anti-apoptotic protein. EphB2 is an ephrin receptor and is up-regulated in invasive tumors but its role needs to be confirmed in further cases of Wilms tumors.

MSX1 may be associated with the invasion capacity of Wilms tumors . RIN1 is a downstream effector of RAS and

Is netrin-1 associated with macrophage infiltration and polarization in human epicardial adipose tissue in coronary artery disease?

Inflammatory activity originating from the epicardial adipose tissue (EAT) may have a role in coronary artery disease (CAD) pathogenesis . The relationship between macrophage infiltration, polarization in the EAT, and netrin-1 gene expression was investigated . CAD patients had higher CD68+ (p=0.005) and CD11c++ macrophages in EAT when compared to the controls . Increased macrophagage infiltration and polarization were associated with higher IL-12 gene expression in the tissue .

Findings suggest a link between enhanced netrin-1 expression in EAT and macrophage infiltration and polarization in patients with CAD.

yes

### Human: Inflammatory activity originating from the epicardial adipose tissue (EAT) may have a role in coronary artery disease (CAD) pathogenesis . The relationship between macrophage infiltration, polarization in the EAT, and netrin-1 gene expression was investigated . CAD patients had higher CD68+ (p=0.005) and CD11c++ macrophages in EAT when compared to the controls . Increased macrophagage infiltration and polarization were associated with higher IL-12 gene expression in the tissue . Is netrin-1 associated with macrophage infiltration and polarization in human epicardial adipose tissue in coronary artery disease?### Assistant: Findings suggest a link between enhanced netrin-1 expression in EAT and macrophage infiltration and polarization in patients with CAD.

Findings suggest a link between enhanced netrin-1 expression in EAT and macrophage infiltration and polarization in patients with CAD .

Do high-grade , non-flow-limiting dissections negatively impact long-term outcome after paclitaxel-coated balloon angioplasty : an additional analysis from the THUNDER study?

Angiograms obtained in the THUNDER study (ClinicalTrials.gov identifier NCT00156624) were analyzed to compare degrees of dissection and angiographic parameters between the control (uncoated balloons, n=43) and treatment (PCBs) groups before and after the intervention and at 6-month follow-up . Patients with severe dissection (grades C, D, or E) especially seemed to benefit from the PCBs, with late lumen loss of 0.4 mm vs. 2.4mm for controls .

The results of this subgroup analysis suggest that patients with dissection following treatment with a paclitaxel-coated balloon have a very acceptable outcome and stent implantation is not necessary as long as the dissection does not result in acute flow limitation.

no

### Human: Angiograms obtained in the THUNDER study (ClinicalTrials.gov identifier NCT00156624) were analyzed to compare degrees of dissection and angiographic parameters between the control (uncoated balloons, n=43) and treatment (PCBs) groups before and after the intervention and at 6-month follow-up . Patients with severe dissection (grades C, D, or E) especially seemed to benefit from the PCBs, with late lumen loss of 0.4 mm vs. 2.4mm for controls . Do high-grade , non-flow-limiting dissections negatively impact long-term outcome after paclitaxel-coated balloon angioplasty : an additional analysis from the THUNDER study?### Assistant: The results of this subgroup analysis suggest that patients with dissection following treatment with a paclitaxel-coated balloon have a very acceptable outcome and stent implantation is not necessary as long as the dissection does not result in acute flow limitation.

The results of this subgroup analysis suggest that patients with dissection following treatment with a paclitaxel-coated balloon have a

Does agmatine induce Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2 -Receptors?

Agmatine produced relaxation in prostate strips pre-contracted with phenylephrine or KCl in a dose-dependent manner . This relaxation was significantly reduced by BU224, a selective I2 imidazoline receptor (IR) blocker, but not by I1 or I3 IR blockers (efaroxan, KU14R), glibenclamide; cyclic AMP (cAMP) phosphodiesterase inhibitor: IBMX; or protein kinase A (PKA) inhibitor: H-89 .

The results suggest that agmatine causes rat prostate relaxation by activation of the I2 IR, which opens KATP channels through cAMP/PKA pathway.

yes

### Human: Agmatine produced relaxation in prostate strips pre-contracted with phenylephrine or KCl in a dose-dependent manner . This relaxation was significantly reduced by BU224, a selective I2 imidazoline receptor (IR) blocker, but not by I1 or I3 IR blockers (efaroxan, KU14R), glibenclamide; cyclic AMP (cAMP) phosphodiesterase inhibitor: IBMX; or protein kinase A (PKA) inhibitor: H-89 . Does agmatine induce Rat Prostate Relaxation through Activation of Peripheral Imidazoline I2 -Receptors?### Assistant: The results suggest that agmatine causes rat prostate relaxation by activation of the I2 IR, which opens KATP channels through cAMP/PKA pathway.

Agmatine causes rat prostate relaxation by activation of the I2 IR, which opens KATP channels through cAMP/PKA

Does pharmacologic disruption of Polycomb Repressive Complex 2 inhibit tumorigenicity and tumor progression in prostate cancer?

Polycomb repressive complex 2 (PRC2) mediates gene silencing through histone H3K27 methylation . PRC2 components are over-expressed in metastatic prostate cancer (PC) and are required for cancer stem cell (CSC) self-renewal . 3-Dezaneplanocinocin-A (DZNeP) is an inhibitor of PRC 2 with broad anticancer activity . We found that specific PRCC2 genes (EED, EZH2, SUZ12) predict poor prognosis in PC .

DZNeP is effective both in vitro and in vivo against PC cells. DZNeP antitumor activity is in part mediated by inhibition of CSC tumorigenic potential.

yes

### Human: Polycomb repressive complex 2 (PRC2) mediates gene silencing through histone H3K27 methylation . PRC2 components are over-expressed in metastatic prostate cancer (PC) and are required for cancer stem cell (CSC) self-renewal . 3-Dezaneplanocinocin-A (DZNeP) is an inhibitor of PRC 2 with broad anticancer activity . We found that specific PRCC2 genes (EED, EZH2, SUZ12) predict poor prognosis in PC . Does pharmacologic disruption of Polycomb Repressive Complex 2 inhibit tumorigenicity and tumor progression in prostate cancer?### Assistant: DZNeP is effective both in vitro and in vivo against PC cells. DZNeP antitumor activity is in part mediated by inhibition of CSC tumorigenic potential.

DZNeP antitumor activity is in part mediated by inhibition of CSC tumorigenic potential .

Is impaired serum capacity to induce cholesterol efflux associated with endothelial dysfunction in type 2 diabetes mellitus?

We investigated whether the capacity of serum to induce cholesterol efflux was associated with endothelial dysfunction in type 2 diabetes . Endothelium-dependent and -independent vasodilation of the brachial artery was measured by high-resolution vascular ultrasound . Serum cholesterol . efflux capacity was lower in diabetic patients than in the . controls (13.6+/-2.5% vs. 14.6 +/-3.4%, respectively, p=0.02), and both endothelium .dependent . vasodicilation (4.9+/2.2%) and . endothelity-independent . vasodyilation were impaired (13+/-4.3%) and (8.8+/4.1%)

Impaired serum cholesterol efflux capacity was associated with endothelial dysfunction independent of other cardiovascular risk factors.

yes

### Human: We investigated whether the capacity of serum to induce cholesterol efflux was associated with endothelial dysfunction in type 2 diabetes . Endothelium-dependent and -independent vasodilation of the brachial artery was measured by high-resolution vascular ultrasound . Serum cholesterol . efflux capacity was lower in diabetic patients than in the . controls (13.6+/-2.5% vs. 14.6 +/-3.4%, respectively, p=0.02), and both endothelium .dependent . vasodicilation (4.9+/2.2%) and . endothelity-independent . vasodyilation were impaired (13+/-4.3%) and (8.8+/4.1%) Is impaired serum capacity to induce cholesterol efflux associated with endothelial dysfunction in type 2 diabetes mellitus?### Assistant: Impaired serum cholesterol efflux capacity was associated with endothelial dysfunction independent of other cardiovascular risk factors.

Impaired serum cholesterol efflux capacity was associated with endothelial dysfunction independent of other cardiovascular risk factors .

Does indoleamine 2,3-dioxygenase mediate inhibition of virus-specific CD8 ( + ) T cell proliferation by human mesenchymal stromal cells?

MSCs with low IDO expression exhibited a reduced suppressive effect on both allo-antigen- and cytomegalovirus (CMV)-specific CD8(+) T-cell proliferation . 1-methyl-DL-tryptophan (1-MT) could partially abrogate the effect . MSC's inhibited CMV-specific CD 8(+) CD8/10 T- cells regardless of the number of passages and the seeding density . The interferon (IFN)-γ level in the culture system was crucial for MSCS to inhibit the proliferation of CMV .

MSCs inhibit virus-specific CD8(+) T-cell proliferation through IFN-γ-induced IDO activity, resolving current conflicting reports on this issue and indicating the potential need for prophylaxis and surveillance of virus infection in patients treated with MSCs. In addition, our study makes a contribution to the development of MSC potency assay for clinical use.

yes

### Human: MSCs with low IDO expression exhibited a reduced suppressive effect on both allo-antigen- and cytomegalovirus (CMV)-specific CD8(+) T-cell proliferation . 1-methyl-DL-tryptophan (1-MT) could partially abrogate the effect . MSC's inhibited CMV-specific CD 8(+) CD8/10 T- cells regardless of the number of passages and the seeding density . The interferon (IFN)-γ level in the culture system was crucial for MSCS to inhibit the proliferation of CMV . Does indoleamine 2,3-dioxygenase mediate inhibition of virus-specific CD8 ( + ) T cell proliferation by human mesenchymal stromal cells?### Assistant: MSCs inhibit virus-specific CD8(+) T-cell proliferation through IFN-γ-induced IDO activity, resolving current conflicting reports on this issue and indicating the potential need for prophylaxis and surveillance of virus infection in patients treated with MSCs. In addition, our study makes a contribution to the development of MSC potency assay for clinical use.

MSCs inhibit virus-specific CD8(+) T-cell proliferation through IFN-γ-induced IDO activity . Study

Are pulmonary Functions Impaired among Carpet Factory Workers : A Spirometric Evaluation?

Nepal is one of the carpet exporter developing countries and still does have many factories within Kathmandu valley . The health hazards especially the status of carpet factory workers from Nepal has not been studied . A cross-sectional comparative study was designed to recruit carpet factory . workers and healthy controls to assess their pulmonary functions . A total of 118 subjects (59 males and 59 females) were recruited (60 carpet factory. workers and 58 controls) Pulmonary function tests were carried out using Medical International Research Spirolab II portable spirometer .

Carpet industry dusts exposure adversely affects pulmonary functions among its workers. The findings significant increase in the FEV1/FVC ratio and decrease in FEV1, FVC, and PEFR suggest that the effects are both restrictive and obstructive patterns of lung disease.

yes

### Human: Nepal is one of the carpet exporter developing countries and still does have many factories within Kathmandu valley . The health hazards especially the status of carpet factory workers from Nepal has not been studied . A cross-sectional comparative study was designed to recruit carpet factory . workers and healthy controls to assess their pulmonary functions . A total of 118 subjects (59 males and 59 females) were recruited (60 carpet factory. workers and 58 controls) Pulmonary function tests were carried out using Medical International Research Spirolab II portable spirometer . Are pulmonary Functions Impaired among Carpet Factory Workers : A Spirometric Evaluation?### Assistant: Carpet industry dusts exposure adversely affects pulmonary functions among its workers. The findings significant increase in the FEV1/FVC ratio and decrease in FEV1, FVC, and PEFR suggest that the effects are both restrictive and obstructive patterns of lung disease.

Carpet industry dusts exposure adversely affects pulmonary functions among its workers . The findings suggest that the effects are both restrictive and obstructive patterns

Is the variant hERG/R148W associated with LQTS a mutation that reduces current density on co-expression with the WT?

A variant of the ether-à-go-go related channel (hERG), p.Arg148Trp (R148W) was found at heterozygous state in two infants who died from sudden infant death syndrome (SIDS), one with documented prolonged QTc and Torsade de Pointes (TdP), and in an adult woman with QTsc >500 ms, atrioventricular block and TdP . This variant was previously reported in cases of severe ventricular arrhythmia but very rarely in control subjects .

Such a decrease in hERG current as evidenced here when co-expressing the hERG/R148W variant with the WT may have predisposed to the observed long QT syndrome and associated TdP. Therefore, the heterozygous carriers of hERG/R148W may be at risk of cardiac sudden death.

yes

### Human: A variant of the ether-à-go-go related channel (hERG), p.Arg148Trp (R148W) was found at heterozygous state in two infants who died from sudden infant death syndrome (SIDS), one with documented prolonged QTc and Torsade de Pointes (TdP), and in an adult woman with QTsc >500 ms, atrioventricular block and TdP . This variant was previously reported in cases of severe ventricular arrhythmia but very rarely in control subjects . Is the variant hERG/R148W associated with LQTS a mutation that reduces current density on co-expression with the WT?### Assistant: Such a decrease in hERG current as evidenced here when co-expressing the hERG/R148W variant with the WT may have predisposed to the observed long QT syndrome and associated TdP. Therefore, the heterozygous carriers of hERG/R148W may be at risk of cardiac sudden death.

Such a decrease in hERG current may have predisposed to the observed long QT syndrome and associated TdP . The heter

Is low level HER2 overexpression associated with rapid tumor cell proliferation and poor prognosis in prostate cancer?

HER2 oncogene is involved in the biology of many different tumor types and serves as a prognostic marker and a therapeutic target in breast cancer . In contrast to breast cancer, studies on Her2 overexpression and gene amplification in prostate cancer have yielded different results . The purpose of this study was to learn more on the prevalence and clinical significance of HER2 amplification and overexposure in the prostate cancer . A tissue microarray containing >2,000 prostate cancers with follow-up data was used .

Low-level Her2 overexpression occurs at relevant frequency in prostate cancer and in the absence of gene amplification. Increased Her2 expression may potentially lead to an aggressive behavior of tumor cells through the stimulation of tumor cell proliferation because Her2 staining was shown to be significantly associated with Ki67 labeling index. These data argue for reconsidering anti-Her2 therapy, possibly with modified approaches.

yes

### Human: HER2 oncogene is involved in the biology of many different tumor types and serves as a prognostic marker and a therapeutic target in breast cancer . In contrast to breast cancer, studies on Her2 overexpression and gene amplification in prostate cancer have yielded different results . The purpose of this study was to learn more on the prevalence and clinical significance of HER2 amplification and overexposure in the prostate cancer . A tissue microarray containing >2,000 prostate cancers with follow-up data was used . Is low level HER2 overexpression associated with rapid tumor cell proliferation and poor prognosis in prostate cancer?### Assistant: Low-level Her2 overexpression occurs at relevant frequency in prostate cancer and in the absence of gene amplification. Increased Her2 expression may potentially lead to an aggressive behavior of tumor cells through the stimulation of tumor cell proliferation because Her2 staining was shown to be significantly associated with Ki67 labeling index. These data argue for reconsidering anti-Her2 therapy, possibly with modified approaches.

Low-level Her2 overexpression occurs at relevant frequency in prostate cancer . Increased Her2 expression may potentially lead to an aggressive behavior

Are disease activity and damage associated with increased levels of fatigue in systemic lupus erythematosus patients from a multiethnic cohort : LXVII?

A total of 515 patients ( approximately 91% female) contributed 2,609 visits to these analyses . Of these patients, 93 (18.1%) were Texan-Hispanic, 101 (19.6%) were Puerto Rican-Hispanic; 169 (32.8%) were African Americans, and 152 (29.5%) were Caucasian . Variables associated with increased levels of fatigue were Caucasian ethnicity, presence of constitutional symptoms (fever, weight loss), and higher levels of pain, abnormal illness-related behaviors, and helplessness .

The presence of pain, abnormal illness-related behaviors, helplessness, and constitutional manifestations were associated with increased levels of fatigue. However, SLE-specific measures, such as disease activity and damage, were not. Interventions aimed at decreasing fatigue need to take into account these findings.

no

### Human: A total of 515 patients ( approximately 91% female) contributed 2,609 visits to these analyses . Of these patients, 93 (18.1%) were Texan-Hispanic, 101 (19.6%) were Puerto Rican-Hispanic; 169 (32.8%) were African Americans, and 152 (29.5%) were Caucasian . Variables associated with increased levels of fatigue were Caucasian ethnicity, presence of constitutional symptoms (fever, weight loss), and higher levels of pain, abnormal illness-related behaviors, and helplessness . Are disease activity and damage associated with increased levels of fatigue in systemic lupus erythematosus patients from a multiethnic cohort : LXVII?### Assistant: The presence of pain, abnormal illness-related behaviors, helplessness, and constitutional manifestations were associated with increased levels of fatigue. However, SLE-specific measures, such as disease activity and damage, were not. Interventions aimed at decreasing fatigue need to take into account these findings.

Pain, abnormal illness-related behaviors, helplessness, and constitutional manifestations were associated with increased levels of fatigue . SLE-specific measures

Is serum BDNF concentration after delivery associated with development of postpartum depression : A 3-month follow up study?

All eligible women not on medication for depression giving birth at the Fourth Affiliated Hospital of Harbin Medical University were consecutively recruited and followed up for 3 months . At 3 months postpartum, women were screened for depression using the Edinburgh Postnatal Depression Scale (EPDS) The primary outcome measure was an EPDS score of > or =12.0ng/ml . Serum BDNF levels in women without PPD were significantly higher than those in women with PPD (P<0.0001)

The present study demonstrates a strong relationship between reduced serum BDNF levels at admission and the development of PPD within the 3 months.

yes

### Human: All eligible women not on medication for depression giving birth at the Fourth Affiliated Hospital of Harbin Medical University were consecutively recruited and followed up for 3 months . At 3 months postpartum, women were screened for depression using the Edinburgh Postnatal Depression Scale (EPDS) The primary outcome measure was an EPDS score of > or =12.0ng/ml . Serum BDNF levels in women without PPD were significantly higher than those in women with PPD (P<0.0001) Is serum BDNF concentration after delivery associated with development of postpartum depression : A 3-month follow up study?### Assistant: The present study demonstrates a strong relationship between reduced serum BDNF levels at admission and the development of PPD within the 3 months.

The present study demonstrates a strong relationship between reduced serum BDNF levels at admission and the development of PPD within the 3 months .

Does sleeve gastrectomy provide a better control of diabetes by decreasing ghrelin in the diabetic Goto-Kakizaki rats?

Sleeve gastrectomy (SG) and modified duodenal jejunal bypass (MDJB) were compared as procedures for glucose control . We aim to form the initial conclusions with respect to the possibility of (1) whether gastric fundus exclusion is essential for the control of diabetes and (2) application as a low morbidity procedure . SG showed strikingly improved blood glucose levels and significantly decreased Ghrelin secretion (P < 0.001) SG resulted in a shorter operative time (P = 0.01) and postoperative recovery time (p < 0 .01) than MDJB group .

SG shows better control in terms of glucose tolerance and other measurements. This study provides direct evidence that SG possesses better improvement of diabetes by reduction of Ghrelin.

yes

### Human: Sleeve gastrectomy (SG) and modified duodenal jejunal bypass (MDJB) were compared as procedures for glucose control . We aim to form the initial conclusions with respect to the possibility of (1) whether gastric fundus exclusion is essential for the control of diabetes and (2) application as a low morbidity procedure . SG showed strikingly improved blood glucose levels and significantly decreased Ghrelin secretion (P < 0.001) SG resulted in a shorter operative time (P = 0.01) and postoperative recovery time (p < 0 .01) than MDJB group . Does sleeve gastrectomy provide a better control of diabetes by decreasing ghrelin in the diabetic Goto-Kakizaki rats?### Assistant: SG shows better control in terms of glucose tolerance and other measurements. This study provides direct evidence that SG possesses better improvement of diabetes by reduction of Ghrelin.

SG shows better control in terms of glucose tolerance and other measurements . This study provides direct evidence that SG possesses better improvement of diabetes by reduction

Is urinary neutrophil gelatinase-associated lipocalin ( NGAL ) associated with mortality in a community-based cohort of older Swedish men?

U-NGAL indicates tubular kidney damage, neutrophil activation and possibly atherogenesis . Study evaluates the association between urinary and serum NGAL and mortality in a Swedish population of 597 men aged 78 years . U- NGAL was associated with increased cardiovascular mortality, independent of other cardiovascular risk factors, but not urinary Albumin (u-Alb) Study found a combination of high u-NGal (≥3 mg/mmol Cr) and high NGAL ( <1.19 μg/mmolec) was associated .

This community study is the first to show that the tubular kidney biomarker u-NGAL associated with increased cardiovascular and all-cause mortality independent of cardiovascular risk factors and glomerular filtration. Additional research is needed to evaluate the utility of NGAL in clinical practice.

yes

### Human: U-NGAL indicates tubular kidney damage, neutrophil activation and possibly atherogenesis . Study evaluates the association between urinary and serum NGAL and mortality in a Swedish population of 597 men aged 78 years . U- NGAL was associated with increased cardiovascular mortality, independent of other cardiovascular risk factors, but not urinary Albumin (u-Alb) Study found a combination of high u-NGal (≥3 mg/mmol Cr) and high NGAL ( <1.19 μg/mmolec) was associated . Is urinary neutrophil gelatinase-associated lipocalin ( NGAL ) associated with mortality in a community-based cohort of older Swedish men?### Assistant: This community study is the first to show that the tubular kidney biomarker u-NGAL associated with increased cardiovascular and all-cause mortality independent of cardiovascular risk factors and glomerular filtration. Additional research is needed to evaluate the utility of NGAL in clinical practice.

The tubular kidney biomarker u-NGAL associated with increased cardiovascular and all-cause mortality independent of cardiovascular risk factors and glomer

Is t-helper 2 immunity associated with reduced risk of myocardial infarction and stroke?

Blood drawn at baseline and incident cardiovascular events during 15-year follow-up were assessed in 700 participants . High numbers of Th2 cells were independently associated with decreased mean common carotid intima-media thickness . No independent associations were found between Th1 cells and CVD risk . Release of the Th2 cytokine interleukin-4 from activated mononuclear leukocytes was independently linked to a reduced risk of CVD. No independent association was found between T-helper (Th) 1 and Th2 .

Our observations provide the first clinical evidence for a protective role of Th2 immunity in CVD. They also suggest this protection is more prominent in women than in men. In spite of convincing evidence from experimental studies, we found no support for a role of Th1 immunity in CVD.

yes

### Human: Blood drawn at baseline and incident cardiovascular events during 15-year follow-up were assessed in 700 participants . High numbers of Th2 cells were independently associated with decreased mean common carotid intima-media thickness . No independent associations were found between Th1 cells and CVD risk . Release of the Th2 cytokine interleukin-4 from activated mononuclear leukocytes was independently linked to a reduced risk of CVD. No independent association was found between T-helper (Th) 1 and Th2 . Is t-helper 2 immunity associated with reduced risk of myocardial infarction and stroke?### Assistant: Our observations provide the first clinical evidence for a protective role of Th2 immunity in CVD. They also suggest this protection is more prominent in women than in men. In spite of convincing evidence from experimental studies, we found no support for a role of Th1 immunity in CVD.

First clinical evidence for a protective role of Th2 immunity in CVD . They also suggest this protection is more prominent in women than in

Does pre-inoculation of urinary catheters with Escherichia coli 83972 inhibit catheter colonization by Enterococcus faecalis?

The capacity of a preexisting coating of Escherichia coli 83972 to reduce catheter colonization by Enterococcus faecalis 210 was investigated . Enterococus was chosen for these trials since it is a common urinary pathogen in patients with an indwelling urinary catheter . A coating of E. coli reduced catheter colonization by E. faecali 210 more than 10-fold . A previous coating of the coating reduced catheters' colonization by Enterococcus more than 10 times .

Pre-inoculating urinary catheters with E. coli 83972 significantly impedes catheter colonization by Enterococcus: These promising in vitro results prompt the clinical investigation of this particular application of bacterial interference.

yes

### Human: The capacity of a preexisting coating of Escherichia coli 83972 to reduce catheter colonization by Enterococcus faecalis 210 was investigated . Enterococus was chosen for these trials since it is a common urinary pathogen in patients with an indwelling urinary catheter . A coating of E. coli reduced catheter colonization by E. faecali 210 more than 10-fold . A previous coating of the coating reduced catheters' colonization by Enterococcus more than 10 times . Does pre-inoculation of urinary catheters with Escherichia coli 83972 inhibit catheter colonization by Enterococcus faecalis?### Assistant: Pre-inoculating urinary catheters with E. coli 83972 significantly impedes catheter colonization by Enterococcus: These promising in vitro results prompt the clinical investigation of this particular application of bacterial interference.

Pre-inoculating urinary catheters with E. coli 83972 significantly impedes catheter colonization by Enterococcus .

Do zygomatic-maxillary buttress reconstruction of midface defects with the osteocutaneous radial forearm free flap?

The purpose of this study was to evaluate morbidity, functional, and aesthetic outcomes in midface zygomatic-maxillary buttress reconstruction using the osteocutaneous radial forearm free flap (OCRFFF) A retrospective review of 24 consecutive patients that underwent midface reconstruction using OCRFFF was performed . There were no flap losses and over 80% of the patients had adequate swallowing, speech, and reincorporation to normal daily activities . The most significant recipient-site complications were severe ectropion (24%), dystopia (8%), and oronasal fistula (12%)

The OCRFFF is an excellent alternative for midface reconstruction of the zygomatic-maxillary buttress. Complications were more common in patients who underwent resection of the orbital rim and floor (type III and IV defects) or external cheek skin.

yes

### Human: The purpose of this study was to evaluate morbidity, functional, and aesthetic outcomes in midface zygomatic-maxillary buttress reconstruction using the osteocutaneous radial forearm free flap (OCRFFF) A retrospective review of 24 consecutive patients that underwent midface reconstruction using OCRFFF was performed . There were no flap losses and over 80% of the patients had adequate swallowing, speech, and reincorporation to normal daily activities . The most significant recipient-site complications were severe ectropion (24%), dystopia (8%), and oronasal fistula (12%) Do zygomatic-maxillary buttress reconstruction of midface defects with the osteocutaneous radial forearm free flap?### Assistant: The OCRFFF is an excellent alternative for midface reconstruction of the zygomatic-maxillary buttress. Complications were more common in patients who underwent resection of the orbital rim and floor (type III and IV defects) or external cheek skin.

Complications were more common in patients who underwent resection of the orbital rim and floor (type III and IV defects) or external cheek

Does blood withdrawn through a cannula valve connector result in clinically significant haemolysis?

Semi-blinded, randomized study paired blood samples, with and without cannula valve connector, were obtained from patients within the ED . Patients were randomized as to which sample was collected first . Overall rate of haemolysis was 2.6%, being 2.8% in the valve first group and 2.5% in no valve first . Rate of hyperkalaemia was not different between valve first and no-valve first groups (12.7% and 13.7%, respectively, P = 1.0)

The attachment of a cannula connector valve to a peripheral cannula prior to blood sampling is not associated with an increase in the rate of haemolysis or hyperkalaemia.

no

### Human: Semi-blinded, randomized study paired blood samples, with and without cannula valve connector, were obtained from patients within the ED . Patients were randomized as to which sample was collected first . Overall rate of haemolysis was 2.6%, being 2.8% in the valve first group and 2.5% in no valve first . Rate of hyperkalaemia was not different between valve first and no-valve first groups (12.7% and 13.7%, respectively, P = 1.0) Does blood withdrawn through a cannula valve connector result in clinically significant haemolysis?### Assistant: The attachment of a cannula connector valve to a peripheral cannula prior to blood sampling is not associated with an increase in the rate of haemolysis or hyperkalaemia.

Attachment of a cannula connector valve to a peripheral cannula prior to blood sampling is not associated with an increase in the rate of

Do long-term intrathecal morphine and bupivacaine upregulate MOR gene expression in lymphocytes?

Opioid receptors may suppress immune system by binding to mu-opioid receptor (MOR) in immune cells . MOR mRNA levels were evaluated in peripheral lymphocytes, and lymphocyte subsets were determined by direct immunofluorescence using flow cytometry . After 12 months of treatment with intrathecal morphine (1.5-4 mg/day), there was an increase in MOR mRNA . levels in lymphocytes of 65% compared with controls and 47% with pretreatment values . Even higher levels (increase of 142%) were observed in the patients treated with morphine plus bupivacaine (0.2-0.4 mg)

This preliminary study suggests that opioids must be used with care in patients who are already immunosuppressed by disease or by other, concurrently administered drugs.

yes

### Human: Opioid receptors may suppress immune system by binding to mu-opioid receptor (MOR) in immune cells . MOR mRNA levels were evaluated in peripheral lymphocytes, and lymphocyte subsets were determined by direct immunofluorescence using flow cytometry . After 12 months of treatment with intrathecal morphine (1.5-4 mg/day), there was an increase in MOR mRNA . levels in lymphocytes of 65% compared with controls and 47% with pretreatment values . Even higher levels (increase of 142%) were observed in the patients treated with morphine plus bupivacaine (0.2-0.4 mg) Do long-term intrathecal morphine and bupivacaine upregulate MOR gene expression in lymphocytes?### Assistant: This preliminary study suggests that opioids must be used with care in patients who are already immunosuppressed by disease or by other, concurrently administered drugs.

This preliminary study suggests that opioids must be used with care in patients who are already immunosuppressed by disease or by other drugs .

Does serum Mac-2 binding protein glycosylation isomer predict grade F4 liver fibrosis in patients with biliary atresia?

Mac-2 Binding Protein Glycosylation Isomer (M2BPGi) is a novel fibrosis marker . We examined the ability of M2Bpgi to predict liver fibrosis in patients with biliary atresia . In comparison with other fibrosis markers such as hyaluronic acid, procollagen-III-peptide, type IV collagen 7s, and aspartate aminotransferase platelet ratio index, M2Pi showed the strongest ability to predict grade F4 fibrosis .

M2BPGi is a novel fibrosis marker for evaluating the status of the liver in patients with BA, especially when predicting grade F4 fibrosis.

yes

### Human: Mac-2 Binding Protein Glycosylation Isomer (M2BPGi) is a novel fibrosis marker . We examined the ability of M2Bpgi to predict liver fibrosis in patients with biliary atresia . In comparison with other fibrosis markers such as hyaluronic acid, procollagen-III-peptide, type IV collagen 7s, and aspartate aminotransferase platelet ratio index, M2Pi showed the strongest ability to predict grade F4 fibrosis . Does serum Mac-2 binding protein glycosylation isomer predict grade F4 liver fibrosis in patients with biliary atresia?### Assistant: M2BPGi is a novel fibrosis marker for evaluating the status of the liver in patients with BA, especially when predicting grade F4 fibrosis.

M2BPGi is a novel fibrosis marker for evaluating the status of the liver in patients with BA, especially when predicting grade

Do breakpoints around the HOXD cluster result in various limb malformations?

Three patients with limb abnormalities and breakpoints involving chromosome 2q31 . None of the three breakpoints disrupted any known genes . None disrupted genes associated with balanced chromosome rearrangements . The study is a promising starting point in the search for candidate genes and regulatory elements . We have mapped the breakpoints to intervals of approximately 170kb or less. None disrupted known genes. The study was published in the journal Nature Biologia, published online on October 1, 2015, and published in The Nature Nature Publishing Group.

Hoxd gene expression in the mouse is regulated by cis-acting DNA elements acting over distances of several hundred kilobases. Moreover, Hoxd genes play an established role in bone development. It is therefore very likely that the three rearrangements disturb normal HOXD gene regulation by position effects.

yes

### Human: Three patients with limb abnormalities and breakpoints involving chromosome 2q31 . None of the three breakpoints disrupted any known genes . None disrupted genes associated with balanced chromosome rearrangements . The study is a promising starting point in the search for candidate genes and regulatory elements . We have mapped the breakpoints to intervals of approximately 170kb or less. None disrupted known genes. The study was published in the journal Nature Biologia, published online on October 1, 2015, and published in The Nature Nature Publishing Group. Do breakpoints around the HOXD cluster result in various limb malformations?### Assistant: Hoxd gene expression in the mouse is regulated by cis-acting DNA elements acting over distances of several hundred kilobases. Moreover, Hoxd genes play an established role in bone development. It is therefore very likely that the three rearrangements disturb normal HOXD gene regulation by position effects.

Hoxd gene expression in the mouse is regulated by cis-acting DNA elements acting over distances of several hundred kilobases . H

Does aldosterone induce endothelial dysfunction in resistance arteries from normotensive and hypertensive rats by increasing thromboxane A2 and prostacyclin?

The study was designed to assess whether cyclooxygenase-2 (COX-2) activation is involved in the effects of chronic aldosterone treatment on endothelial function of mesenteric resistance arteries (MRA) from Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR) Relaxation to acetylcholine was measured in MRA from both untreated and ald testosterone-treated strains . Release of 6-oxo-prostaglandin (PG)F1alpha and thromboxane B2 (TxB2) was determined by enzyme immunoassay .

Chronic treatment with aldosterone impaired endothelial function in MRA under normotensive and hypertensive conditions by increasing COX-2-derived prostacyclin and thromboxane A2. As endothelial dysfunction participates in the pathogenesis of many cardiovascular disorders we hypothesize that anti-inflammatory drugs, specifically COX-2 inhibitors, could ameliorate vascular damage in patients with elevated aldosterone production.

yes

### Human: The study was designed to assess whether cyclooxygenase-2 (COX-2) activation is involved in the effects of chronic aldosterone treatment on endothelial function of mesenteric resistance arteries (MRA) from Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR) Relaxation to acetylcholine was measured in MRA from both untreated and ald testosterone-treated strains . Release of 6-oxo-prostaglandin (PG)F1alpha and thromboxane B2 (TxB2) was determined by enzyme immunoassay . Does aldosterone induce endothelial dysfunction in resistance arteries from normotensive and hypertensive rats by increasing thromboxane A2 and prostacyclin?### Assistant: Chronic treatment with aldosterone impaired endothelial function in MRA under normotensive and hypertensive conditions by increasing COX-2-derived prostacyclin and thromboxane A2. As endothelial dysfunction participates in the pathogenesis of many cardiovascular disorders we hypothesize that anti-inflammatory drugs, specifically COX-2 inhibitors, could ameliorate vascular damage in patients with elevated aldosterone production.

Anti-inflammatory drugs, specifically COX-2 inhibitors, could ameliorate vascular damage in patients with elevated aldosterone production

Are red blood cell n-3 polyunsaturated fatty acids in first trimester of pregnancy inversely associated with placental weight?

Red blood cell (RBC) level of long-chain n-3 polyunsaturated fatty acids (PUFA) in the first trimester of pregnancy . Placental weight was the only one associated with long-chilled n-2 PUFA in RBC . Inverse association was found between the proportion of PUFA and placental weight, adjusted for birthweight (p=0.035) Inverse associations were found between long-chains n-1 PUFA intake and placetic weight .

The human fetus relies on maternal supply and placental delivery of long-chain n-3 PUFA for optimal development and function, particularly of the central nervous system. Given the importance of dietary n-3 PUFA during pregnancy, further studies are warranted to investigate the relationship between placental weight, maternal long-chain n-3 PUFA status and smoking.

yes

### Human: Red blood cell (RBC) level of long-chain n-3 polyunsaturated fatty acids (PUFA) in the first trimester of pregnancy . Placental weight was the only one associated with long-chilled n-2 PUFA in RBC . Inverse association was found between the proportion of PUFA and placental weight, adjusted for birthweight (p=0.035) Inverse associations were found between long-chains n-1 PUFA intake and placetic weight . Are red blood cell n-3 polyunsaturated fatty acids in first trimester of pregnancy inversely associated with placental weight?### Assistant: The human fetus relies on maternal supply and placental delivery of long-chain n-3 PUFA for optimal development and function, particularly of the central nervous system. Given the importance of dietary n-3 PUFA during pregnancy, further studies are warranted to investigate the relationship between placental weight, maternal long-chain n-3 PUFA status and smoking.

The human fetus relies on maternal supply and placental delivery of long-chain n-3 PUFA for optimal development and function, particularly

Does increased expression of HMGA1 correlate with tumour invasiveness and proliferation in human pituitary adenomas?

High-mobility group A1 (HMGA1) is highly expressed in various benign and malignant tumours . HMGA1 expression was frequently detected in clinically non-functioning adenomas . It was also detected in 48.1% of growth hormone (GH), 60% of mixed GH/prolactin (PRL), 62.5% of PRL, 66.6% of thyroid-stimulating hormone (THTH) and 37.5%) adenoma .

These findings suggest that HMGA1 up-regulation has an important oncogenic role in pituitary tumorigenesis, as well as being a novel molecular marker of tumour proliferation and invasiveness.

yes

### Human: High-mobility group A1 (HMGA1) is highly expressed in various benign and malignant tumours . HMGA1 expression was frequently detected in clinically non-functioning adenomas . It was also detected in 48.1% of growth hormone (GH), 60% of mixed GH/prolactin (PRL), 62.5% of PRL, 66.6% of thyroid-stimulating hormone (THTH) and 37.5%) adenoma . Does increased expression of HMGA1 correlate with tumour invasiveness and proliferation in human pituitary adenomas?### Assistant: These findings suggest that HMGA1 up-regulation has an important oncogenic role in pituitary tumorigenesis, as well as being a novel molecular marker of tumour proliferation and invasiveness.

HMGA1 up-regulation has an important oncogenic role in pituitary tumorigenesis, as well as being a novel

Does telmisartan prevent VCAM-1 induction and monocytic cell adhesion to endothelium exposed to non-uniform shear stress and TNF-α?

Telmisartan, an anti-inflammatory angiotensin II receptor blocker with PPAR-gamma activating ability, prevents monocyte recruitment by endothelium . The inhibitory effect of telmisaran on cell adhesion was prevented in the presence of GW9662 . The effect was mediated by a significant reduction of endothelial VCAM-1 expression . On the contrary, the induction of E-selectin by TNF-alpha in ECs exposed to non-uniform shear stress was not affected .

Treatment with telmisartan decreases the TNF-α-induced recruitment of monocytic cells and endothelial expression of VCAM-1 in regions of non-uniform shear stress in vitro. This mechanism can contribute to the beneficial pleiotropic effects of telmisartan in atherosclerosis-prone arterial regions.

yes

### Human: Telmisartan, an anti-inflammatory angiotensin II receptor blocker with PPAR-gamma activating ability, prevents monocyte recruitment by endothelium . The inhibitory effect of telmisaran on cell adhesion was prevented in the presence of GW9662 . The effect was mediated by a significant reduction of endothelial VCAM-1 expression . On the contrary, the induction of E-selectin by TNF-alpha in ECs exposed to non-uniform shear stress was not affected . Does telmisartan prevent VCAM-1 induction and monocytic cell adhesion to endothelium exposed to non-uniform shear stress and TNF-α?### Assistant: Treatment with telmisartan decreases the TNF-α-induced recruitment of monocytic cells and endothelial expression of VCAM-1 in regions of non-uniform shear stress in vitro. This mechanism can contribute to the beneficial pleiotropic effects of telmisartan in atherosclerosis-prone arterial regions.

Telmisartan decreases the TNF-α-induced recruitment of monocytic cells and endothelial expression of VCAM-1

Do guidelines do help change behavior in the management of osteoporosis by gastroenterologists?

The American College of Gastroenterology supported the development of evidence-based guidelines for the management of osteoporosis in inflammatory bowel disease . The number of bone density scans ordered by these physicians increased by 100% in these practices after the guidelines were disseminated . There was a significant number of subjects who felt more comfortable treating osteoporeosis (20%vs 10% prior to dissemination of the guidelines [p]]. There was also a 150% increase in the number of patients who had received bone density scan orders by these practitioners .

Although a minority of subjects read the guidelines, there was a significant impact on the short-term practice patterns of these practitioners.

yes

### Human: The American College of Gastroenterology supported the development of evidence-based guidelines for the management of osteoporosis in inflammatory bowel disease . The number of bone density scans ordered by these physicians increased by 100% in these practices after the guidelines were disseminated . There was a significant number of subjects who felt more comfortable treating osteoporeosis (20%vs 10% prior to dissemination of the guidelines [p]]. There was also a 150% increase in the number of patients who had received bone density scan orders by these practitioners . Do guidelines do help change behavior in the management of osteoporosis by gastroenterologists?### Assistant: Although a minority of subjects read the guidelines, there was a significant impact on the short-term practice patterns of these practitioners.

Although a minority of subjects read the guidelines, there was a significant impact on the short-term practice patterns of these practitioners .

Does bactericidal activity predict sterilizing activity : the case of rifapentine in the murine model of Mycobacterium ulcerans disease?

Daily treatment with streptomycin (STR) + rifampin (RIF) for 8 weeks has shifted from surgery to daily treatment . We tested the potential of daily rifapentine (RPT), a long-acting rifamycin derivative, as a substitute for RIF . All drug treatments were bactericidal. After 1 week of treatment, the decline in CFU counts was significantly greater in treated mice . After 2 weeks of treatment the decline was greater in mice treated with STR+RPT .

The better bactericidal activity of daily STR+RIF and especially of STR+RPT did not translate into better prevention of relapse, possibly because relapse-freecure after treatment of Buruli ulcer is more related to the reversal of mycolactone-induced local immunodeficiency by drug treatment rather than to the bactericidal potency of drugs.

no

### Human: Daily treatment with streptomycin (STR) + rifampin (RIF) for 8 weeks has shifted from surgery to daily treatment . We tested the potential of daily rifapentine (RPT), a long-acting rifamycin derivative, as a substitute for RIF . All drug treatments were bactericidal. After 1 week of treatment, the decline in CFU counts was significantly greater in treated mice . After 2 weeks of treatment the decline was greater in mice treated with STR+RPT . Does bactericidal activity predict sterilizing activity : the case of rifapentine in the murine model of Mycobacterium ulcerans disease?### Assistant: The better bactericidal activity of daily STR+RIF and especially of STR+RPT did not translate into better prevention of relapse, possibly because relapse-freecure after treatment of Buruli ulcer is more related to the reversal of mycolactone-induced local immunodeficiency by drug treatment rather than to the bactericidal potency of drugs.

The better bactericidal activity of daily STR+RIF did not translate into better prevention of relapse . relapse-freecure after treatment

Do changes in mineral composition of rabbit corneas after alkali burn?

Thirty-two eyes of rabbits were burnt for 30 s with 1 M NaOH . Sodium was present in high concentrations immediately after burning, up to 708 mmol/kg dry weight . Chlorine increased significantly throughout the whole period . Sulphur concentrations stayed almost normal, showing only a minute loss of ground substance after alkali burn . A considerable and increasing edema could be concluded from the water content. A considerable . and increasing . edema . could be . concluded from . the water . content . The mineral composition of the corneal stroma changed considerably .

Alkali burns resulted in a dramatic change of the corneal mineral content. Therapeutic intervention with appropriate solutions is required to restore and maintain the normal mineral composition of the denuded corneal stroma.

yes

### Human: Thirty-two eyes of rabbits were burnt for 30 s with 1 M NaOH . Sodium was present in high concentrations immediately after burning, up to 708 mmol/kg dry weight . Chlorine increased significantly throughout the whole period . Sulphur concentrations stayed almost normal, showing only a minute loss of ground substance after alkali burn . A considerable and increasing edema could be concluded from the water content. A considerable . and increasing . edema . could be . concluded from . the water . content . The mineral composition of the corneal stroma changed considerably . Do changes in mineral composition of rabbit corneas after alkali burn?### Assistant: Alkali burns resulted in a dramatic change of the corneal mineral content. Therapeutic intervention with appropriate solutions is required to restore and maintain the normal mineral composition of the denuded corneal stroma.

Alkali burns resulted in a dramatic change of the corneal mineral content . Therapeutic intervention with appropriate solutions is required to

Do regulatory T cells inhibit Th1 cell-mediated bile duct injury in murine biliary atresia?

Biliary atresia (BA) is a pediatric inflammatory disease of the biliary system which leads to cirrhosis and the need for liver transplantation . The aim of this study was to determine if Tregs are dysfunctional in the rotavirus-induced mouse model of BA (murine BA) BA resulted from infection of BALB/c neonates with Rhesus rotav virus (RRV) BA mice were decreased in number, activation marker expression, and suppressive function .

These studies demonstrate that dysregulation of Tregs is present in murine BA and that diminished Treg function may be implicated in the pathogenesis of human BA.

yes

### Human: Biliary atresia (BA) is a pediatric inflammatory disease of the biliary system which leads to cirrhosis and the need for liver transplantation . The aim of this study was to determine if Tregs are dysfunctional in the rotavirus-induced mouse model of BA (murine BA) BA resulted from infection of BALB/c neonates with Rhesus rotav virus (RRV) BA mice were decreased in number, activation marker expression, and suppressive function . Do regulatory T cells inhibit Th1 cell-mediated bile duct injury in murine biliary atresia?### Assistant: These studies demonstrate that dysregulation of Tregs is present in murine BA and that diminished Treg function may be implicated in the pathogenesis of human BA.

These studies demonstrate that dysregulation of Tregs is present in murine BA and that diminished Treg function may be implicated in the

Is tPH1 A218 allele associated with suicidal behavior in Turkish population?

The first and rate-limiting step in the synthesis of serotonin is catalyzed by tryptophan hydroxylase (TPH) which is encoded by TPH1 and THP2 genes . Serotonergic dysfunction is implicated in depression, psychiatric disorders and suicidal behaviors . In this study, we investigated A218C polymorphism in 109 suicide attempters and 98 healthy controls . There were no differences in genotype frequencies of this locus between participants having attempted suicide and controls .

Our results provide evidence that A allele of TPH1 A218C polymorphism may be associated with suicidal behavior in Turkish population.

yes

### Human: The first and rate-limiting step in the synthesis of serotonin is catalyzed by tryptophan hydroxylase (TPH) which is encoded by TPH1 and THP2 genes . Serotonergic dysfunction is implicated in depression, psychiatric disorders and suicidal behaviors . In this study, we investigated A218C polymorphism in 109 suicide attempters and 98 healthy controls . There were no differences in genotype frequencies of this locus between participants having attempted suicide and controls . Is tPH1 A218 allele associated with suicidal behavior in Turkish population?### Assistant: Our results provide evidence that A allele of TPH1 A218C polymorphism may be associated with suicidal behavior in Turkish population.

A allele of TPH1 A218C polymorphism may be associated with suicidal behavior in Turkish population .

Does the effect of ankle brace on the prevention of dynamic forced ankle inversion?

Fourteen healthy men with a mean age of 25.1 years were tested . Three braces, two semirigid (Aircast and Bledsoe) and one lace-up (Swede-O) were fitted to each subject . Forced dynamic ankle inversion of 24 degrees was to be resisted as subjects landed on one foot with a force of two body weights on a stimulus presented randomly in 5 of 15 jump trial blocks . All three braces increased the success rate (average, 44%) However, only the two semirsirigids proved to be significantly better than the unbraced state .

This test holds promise for evaluating brace efficacy when landing with one foot unexpectedly on an object that acts to forcibly invert the ankle.

yes

### Human: Fourteen healthy men with a mean age of 25.1 years were tested . Three braces, two semirigid (Aircast and Bledsoe) and one lace-up (Swede-O) were fitted to each subject . Forced dynamic ankle inversion of 24 degrees was to be resisted as subjects landed on one foot with a force of two body weights on a stimulus presented randomly in 5 of 15 jump trial blocks . All three braces increased the success rate (average, 44%) However, only the two semirsirigids proved to be significantly better than the unbraced state . Does the effect of ankle brace on the prevention of dynamic forced ankle inversion?### Assistant: This test holds promise for evaluating brace efficacy when landing with one foot unexpectedly on an object that acts to forcibly invert the ankle.

This test holds promise for evaluating brace efficacy when landing with one foot unexpectedly on an object that acts to forcibly invert the ankle .

Does toll-like receptor 9 promote cardiac inflammation and heart failure during polymicrobial sepsis?

Sepsis was induced via colon ascendens stent peritonitis (CASP) in C57BL/6 wild-type mice . CASP led to continuous release of bacteria into the peritoneal cavity, an increase of cytokines, and differential regulation of receptors of innate immunity in the heart . Eighteen hours after CASP WT mice developed septic heart failure characterised by reduction of end-systolic pressure, stroke volume, cardiac output, and parameters of contractility .

In polymicrobial sepsis TLR9 signalling is pivotal to cardiac inflammation and septic heart failure.

yes

### Human: Sepsis was induced via colon ascendens stent peritonitis (CASP) in C57BL/6 wild-type mice . CASP led to continuous release of bacteria into the peritoneal cavity, an increase of cytokines, and differential regulation of receptors of innate immunity in the heart . Eighteen hours after CASP WT mice developed septic heart failure characterised by reduction of end-systolic pressure, stroke volume, cardiac output, and parameters of contractility . Does toll-like receptor 9 promote cardiac inflammation and heart failure during polymicrobial sepsis?### Assistant: In polymicrobial sepsis TLR9 signalling is pivotal to cardiac inflammation and septic heart failure.

TLR9 signalling is pivotal to cardiac inflammation and septic heart failure . In polymicrobial sepsis TLR 9 signalling is

Does maternal obesity affect fetal myocardial function as early as in the first trimester?

Animal studies have shown that maternal obesity induces fibrosis in fetal myocardium . We hypothesized that fetal cardiac function would be impaired among obese pregnant women . Case-control study with longitudinal follow-up was performed at Trondheim University Hospital, Norway . The main outcome measures were global strain rate (GSR) and strain by tissue Doppler imaging, tissue velocities (TDVs) and interventricular septal thickness assessed by fetal echocardiography at gestational ages of 14, 20 and 32 weeks .

At 14 weeks of gestation, we detected fetal myocardial dysfunction with reduced LV and RV GSR and strain in FOW compared with fetuses of women with normal weight. Our finding is alarming considering the high prevalence of obesity and may partly explain the predisposition of offspring to cardiovascular disease later in life.

yes

### Human: Animal studies have shown that maternal obesity induces fibrosis in fetal myocardium . We hypothesized that fetal cardiac function would be impaired among obese pregnant women . Case-control study with longitudinal follow-up was performed at Trondheim University Hospital, Norway . The main outcome measures were global strain rate (GSR) and strain by tissue Doppler imaging, tissue velocities (TDVs) and interventricular septal thickness assessed by fetal echocardiography at gestational ages of 14, 20 and 32 weeks . Does maternal obesity affect fetal myocardial function as early as in the first trimester?### Assistant: At 14 weeks of gestation, we detected fetal myocardial dysfunction with reduced LV and RV GSR and strain in FOW compared with fetuses of women with normal weight. Our finding is alarming considering the high prevalence of obesity and may partly explain the predisposition of offspring to cardiovascular disease later in life.

At 14 weeks of gestation, fetal myocardial dysfunction with reduced LV and RV GSR and strain in FOW compared with fetuses

Is efficacy of etanercept in combination with methotrexate in moderate-to-severe rheumatoid arthritis dependent on methotrexate dosage?

Post hoc analysis of methotrexate (MTX) dosage on clinical, functional and quality of life outcomes in patients with rheumatoid arthritis (RA) from two previous etanercept (ETN) trials after 24 months of treatment . Patients with active RA in the ETN+MTX combination treatment arms of the Trial of Etanerce and Methotrexate with Radiographic Patient Outcomes (TEMPO) and COmbination of Methotxate and ETanercercept in Active Early Rheumatoids Arthritis (COMET) studies were pooled in this post hoc analysis .

Patients with RA in the TEMPO and COMET trials who received ETN+MTX showed similar efficacy outcomes at 24 months, regardless of MTX dosage.

no

### Human: Post hoc analysis of methotrexate (MTX) dosage on clinical, functional and quality of life outcomes in patients with rheumatoid arthritis (RA) from two previous etanercept (ETN) trials after 24 months of treatment . Patients with active RA in the ETN+MTX combination treatment arms of the Trial of Etanerce and Methotrexate with Radiographic Patient Outcomes (TEMPO) and COmbination of Methotxate and ETanercercept in Active Early Rheumatoids Arthritis (COMET) studies were pooled in this post hoc analysis . Is efficacy of etanercept in combination with methotrexate in moderate-to-severe rheumatoid arthritis dependent on methotrexate dosage?### Assistant: Patients with RA in the TEMPO and COMET trials who received ETN+MTX showed similar efficacy outcomes at 24 months, regardless of MTX dosage.

Patients with RA in the TEMPO and COMET trials who received ETN+MTX showed similar efficacy outcomes at 24-months

Does exposure to dietary lipid lead to rapid production of cytosolic lipid droplets near the brush border membrane?

Intestinal absorption of dietary lipids involves hydrolysis in the lumen of proximal intestine as well as uptake, intracellular transport and re-assembly of hydrolyzed lipids in enterocytes . The enzyme monoacylglycerol acyltransferase 2 (MGAT2) was identified in the brush border membrane (BBM) in addition to the endoplasmic reticulum, suggesting local synthesis of triglycerides and CLD at both places . Intestinal lipid absorption was studied in mouse after gavage .

We show a very fast production of CLD by enterocytes associated with a transfer of apical constituents as lipid transporters. Our findings suggest that following their uptake by enterocytes, lipids can be partially metabolized at the BBM and packaged into CLD for their transportation to the ER.

yes

### Human: Intestinal absorption of dietary lipids involves hydrolysis in the lumen of proximal intestine as well as uptake, intracellular transport and re-assembly of hydrolyzed lipids in enterocytes . The enzyme monoacylglycerol acyltransferase 2 (MGAT2) was identified in the brush border membrane (BBM) in addition to the endoplasmic reticulum, suggesting local synthesis of triglycerides and CLD at both places . Intestinal lipid absorption was studied in mouse after gavage . Does exposure to dietary lipid lead to rapid production of cytosolic lipid droplets near the brush border membrane?### Assistant: We show a very fast production of CLD by enterocytes associated with a transfer of apical constituents as lipid transporters. Our findings suggest that following their uptake by enterocytes, lipids can be partially metabolized at the BBM and packaged into CLD for their transportation to the ER.

We show a very fast production of CLD by enterocytes associated with transfer of apical constituents as lipid transporters . Our findings

Is lymphangiogenesis a predictor of nodal metastasis in extramammary Paget 's disease?

The depth of dermal invasion, lymphatic invasion and tumour formation are thought to be predictors of nodal metastasis in extramammary Paget's disease (EPD) This study investigated the relationship between lymphangiogenesis and nodal metastasis in EPD . Lymphatic invasion was evaluated by D2-40 immunostain and lymphatic invitation was evaluated by immunostains . There were significant differences in the presence or absence of . the presence of . dermal . invasion, depth of invasion, . lymphatic . invasion and . lymphatics invasion were evaluated by . D2

Dermal invasion and depth of dermal invasion are predictors for nodal metastasis in EPD. However, in the current study, we demonstrate that lymphangiogenesis is also a predictor of nodal metastasis in EPD.

yes

### Human: The depth of dermal invasion, lymphatic invasion and tumour formation are thought to be predictors of nodal metastasis in extramammary Paget's disease (EPD) This study investigated the relationship between lymphangiogenesis and nodal metastasis in EPD . Lymphatic invasion was evaluated by D2-40 immunostain and lymphatic invitation was evaluated by immunostains . There were significant differences in the presence or absence of . the presence of . dermal . invasion, depth of invasion, . lymphatic . invasion and . lymphatics invasion were evaluated by . D2 Is lymphangiogenesis a predictor of nodal metastasis in extramammary Paget 's disease?### Assistant: Dermal invasion and depth of dermal invasion are predictors for nodal metastasis in EPD. However, in the current study, we demonstrate that lymphangiogenesis is also a predictor of nodal metastasis in EPD.

Dermal invasion and depth of dermal invasion are predictors for nodal metastasis in EPD . lymphangiogenesis is also

Does an epidemiologic study of closed emergency department malpractice claim in a national database of physician malpractice insurers?

Emergency physicians were the primary defendants in 19% of ED claims . Acute myocardial infarction (AMI; 5%), fractures (6%), and appendicitis (2%) were the health conditions associated with the highest number of claims . The authors identified 11,529 claims arising from an event originating in an ED, representing over $664 million in total liability over the 23-year study period . Over time, the average indemnity payments and expenses of litigation, adjusted for inflation, more than doubled .

Emergency physicians were the primary defendants in a relatively small proportion of ED claims. The disease processes associated with the highest numbers of claims included AMI, appendicitis, and fractures. The largest share of overall indemnity was attributed to errors in the diagnostic process. The financial liability of medical malpractice in the ED is substantial, yet the vast majority of claims resolve in favor of the clinician. Efforts to mitigate risk in the ED should include the diverse clinical specialties who work in this complex environment, with attention to those health conditions and potential errors with the highest risk.

yes

### Human: Emergency physicians were the primary defendants in 19% of ED claims . Acute myocardial infarction (AMI; 5%), fractures (6%), and appendicitis (2%) were the health conditions associated with the highest number of claims . The authors identified 11,529 claims arising from an event originating in an ED, representing over $664 million in total liability over the 23-year study period . Over time, the average indemnity payments and expenses of litigation, adjusted for inflation, more than doubled . Does an epidemiologic study of closed emergency department malpractice claim in a national database of physician malpractice insurers?### Assistant: Emergency physicians were the primary defendants in a relatively small proportion of ED claims. The disease processes associated with the highest numbers of claims included AMI, appendicitis, and fractures. The largest share of overall indemnity was attributed to errors in the diagnostic process. The financial liability of medical malpractice in the ED is substantial, yet the vast majority of claims resolve in favor of the clinician. Efforts to mitigate risk in the ED should include the diverse clinical specialties who work in this complex environment, with attention to those health conditions and potential errors with the highest risk.

Emergency physicians were the primary defendants in a relatively small proportion of ED claims . The disease processes associated with the highest numbers of claims included AM

Does cytosolic sequestration of Prep1 influence early stages of T cell development?

Prep1 and Pbx2 are the main homeodomain DNA-binding proteins of the TALE (three amino acid loop extension) family expressed in the thymus . We previously reported reduced PbX2 expression and defective thymocyte maturation in Prep1 hypomorphic mice . We generated transgenic mice expressing the N-terminal fragment of Pb x1 (Pbx1NT) under the control of the Lck proximal promoter . Transgenic thymi reveal increased numbers of CD4(-) CD8(-)CD44(-) (DN3 and DN4) thymocytes .

The results obtained by genetic (Prep1 hypomorphic) and functional (Pbx1NT transgenic) inactivation of Prep1 support nonredundant roles for this homeodomain protein during different stages of T cell development.

yes

### Human: Prep1 and Pbx2 are the main homeodomain DNA-binding proteins of the TALE (three amino acid loop extension) family expressed in the thymus . We previously reported reduced PbX2 expression and defective thymocyte maturation in Prep1 hypomorphic mice . We generated transgenic mice expressing the N-terminal fragment of Pb x1 (Pbx1NT) under the control of the Lck proximal promoter . Transgenic thymi reveal increased numbers of CD4(-) CD8(-)CD44(-) (DN3 and DN4) thymocytes . Does cytosolic sequestration of Prep1 influence early stages of T cell development?### Assistant: The results obtained by genetic (Prep1 hypomorphic) and functional (Pbx1NT transgenic) inactivation of Prep1 support nonredundant roles for this homeodomain protein during different stages of T cell development.

Prep1 has nonredundant roles for this homeodomain protein during different stages of T cell development . The results obtained by

Do aging-related changes in cutaneous corticotropin-releasing hormone system reflect a defective neuroendocrine-stress response in aging?

Aging is a complex process that involves the decline of function or dysfunction of many systems . The corticotropin-releasing hormone (CRH) system is involved in skin inflammation . CRH has a suggested role in age-associated conditions and in animal aging models . A consistent logic interaction between the different CRH system components and the aging process has never been examined before . Age-associated downregulation of CRHBP in the sebaceous and sweat glands was detected, whereas the CRHR2 showed no age-related changes .

Our findings suggest that the age-associated changes in the expression of CRH system components reflect an exaggerated stress response reaction, putting the aged skin continuously in a stress-like situation.

yes

### Human: Aging is a complex process that involves the decline of function or dysfunction of many systems . The corticotropin-releasing hormone (CRH) system is involved in skin inflammation . CRH has a suggested role in age-associated conditions and in animal aging models . A consistent logic interaction between the different CRH system components and the aging process has never been examined before . Age-associated downregulation of CRHBP in the sebaceous and sweat glands was detected, whereas the CRHR2 showed no age-related changes . Do aging-related changes in cutaneous corticotropin-releasing hormone system reflect a defective neuroendocrine-stress response in aging?### Assistant: Our findings suggest that the age-associated changes in the expression of CRH system components reflect an exaggerated stress response reaction, putting the aged skin continuously in a stress-like situation.

Age-associated changes in the expression of CRH system components reflect an exaggerated stress response reaction, putting the aged skin continuously in a stress

Is number of islet autoantibodies present in newly diagnosed type 1 diabetes children born to non-diabetic mothers affected by islet autoantibodies present at birth?

Islet autoantibodies at birth and at diagnosis may be associated with a reduced risk of islet autoimmunity and diabetes . Children born to non-diabetic mothers with type 1 diabetes may be at a lower risk of developing type 1 and diabetes, study found . Children with fewer islet . autoantigodies at diagnosis were more likely to have had auto antibodies . at birth than at diagnosis, and in only 5% (4/86) of children with more than two antibodies .

Our preliminary data suggest that exposure to cord blood islet autoantibodies may influence the presence of islet autoantibodies at the time of diagnosis of type 1 diabetes and explain why some type 1 diabetes children are islet autoantibody negative at clinical diagnosis.

yes

### Human: Islet autoantibodies at birth and at diagnosis may be associated with a reduced risk of islet autoimmunity and diabetes . Children born to non-diabetic mothers with type 1 diabetes may be at a lower risk of developing type 1 and diabetes, study found . Children with fewer islet . autoantigodies at diagnosis were more likely to have had auto antibodies . at birth than at diagnosis, and in only 5% (4/86) of children with more than two antibodies . Is number of islet autoantibodies present in newly diagnosed type 1 diabetes children born to non-diabetic mothers affected by islet autoantibodies present at birth?### Assistant: Our preliminary data suggest that exposure to cord blood islet autoantibodies may influence the presence of islet autoantibodies at the time of diagnosis of type 1 diabetes and explain why some type 1 diabetes children are islet autoantibody negative at clinical diagnosis.

Exposure to cord blood islet autoantibodies may influence the presence of islet Autoantibody at the time of diagnosis of

Is variation in catechol-O-methyltransferase associated with duloxetine response in a clinical trial for major depressive disorder?

The study objective was to evaluate variations in genes implicated in antidepressant mechanism of action for association with response to duloxetine treatment in major depressive disorder (MDD) We assessed response over 6 weeks in 250 patients in a randomized, double-blind study of patients with MDD . Peak association was detected with rs165599 (p = .006), which accounted for approximately 3% of variance in HAMD17 change and >4% of variances in IDS-C-30 change . For SNPs in serotonin 2A receptor (HTR2A) previously associated with citalopram response, including rs7997012, including .

Single nucleotide polymorphisms in COMT were associated with symptom change in duloxetine-treated patients with MDD. If replicated, the magnitude of the COMT genotype effect is of clinical relevance.

yes

### Human: The study objective was to evaluate variations in genes implicated in antidepressant mechanism of action for association with response to duloxetine treatment in major depressive disorder (MDD) We assessed response over 6 weeks in 250 patients in a randomized, double-blind study of patients with MDD . Peak association was detected with rs165599 (p = .006), which accounted for approximately 3% of variance in HAMD17 change and >4% of variances in IDS-C-30 change . For SNPs in serotonin 2A receptor (HTR2A) previously associated with citalopram response, including rs7997012, including . Is variation in catechol-O-methyltransferase associated with duloxetine response in a clinical trial for major depressive disorder?### Assistant: Single nucleotide polymorphisms in COMT were associated with symptom change in duloxetine-treated patients with MDD. If replicated, the magnitude of the COMT genotype effect is of clinical relevance.

Single nucleotide polymorphisms in COMT were associated with symptom change in duloxetine-treated patients with MDD . If

Is early postoperative reduction of monoclonal antimyosin antibody uptake associated with absent rejection-related complications after heart transplantation?

111In-labeled monoclonal antimyosin antibodies (MAA) detected rejection activity detected by biopsy . Relationship between degree of uptake and the occurrence of severe rejection-related complications has not been previously assessed . No complications were noted in 193 studies of patients with HLR of less than 2.00, whereas in nine of 45 with HRL of 2 .00 or greater, complications occurred in 9 of 45 patients . None of the 23 patients prospectively followed since surgery who had a gradual decrease in MAA uptake during the first 3 months showed rejection- related complications, whereas persistent uptake was associated with complications .

No rejection-related complications are seen coinciding with HLR of less than 2.00, whereas patients who have complications have an HLR of more than 2.00. The early 3-month pattern of decreasing MAA uptake is associated with a clinical course free of rejection-related complications, whereas a persistent pattern is a signal of the possibility of such complications.

yes

### Human: 111In-labeled monoclonal antimyosin antibodies (MAA) detected rejection activity detected by biopsy . Relationship between degree of uptake and the occurrence of severe rejection-related complications has not been previously assessed . No complications were noted in 193 studies of patients with HLR of less than 2.00, whereas in nine of 45 with HRL of 2 .00 or greater, complications occurred in 9 of 45 patients . None of the 23 patients prospectively followed since surgery who had a gradual decrease in MAA uptake during the first 3 months showed rejection- related complications, whereas persistent uptake was associated with complications . Is early postoperative reduction of monoclonal antimyosin antibody uptake associated with absent rejection-related complications after heart transplantation?### Assistant: No rejection-related complications are seen coinciding with HLR of less than 2.00, whereas patients who have complications have an HLR of more than 2.00. The early 3-month pattern of decreasing MAA uptake is associated with a clinical course free of rejection-related complications, whereas a persistent pattern is a signal of the possibility of such complications.

No rejection-related complications are seen coinciding with HLR of less than 2.00, whereas patients who have complications have an H

Does anatomic double-bundle ACL reconstruction restrict knee extension in knees with hyperextension?

Until now, the influence on knees with hyperextension has not been evaluated . Study evaluated whether double-bundle ACL reconstruction restricts extension in hypereextendable knees . The knee extension was 4°± 1.8° with the intact ACL and 4° ±  1.7° after reconstruction in the SB group (n.s) The limitation of the extension was significantly larger in the DB group (p = 0.013) The difference in AP translation before and after the reconstruction was not significantly different in 30° and 90° of flexion .

Both single- and double-bundle ACL reconstruction techniques are capable of restoring knee anteroposterior and rotational stability. Double-bundle reconstructions significantly reduce knee extension in knees with hyperextension capability. Care must be taken when using double-bundle techniques in patients with knee hyperextension as this procedure may limit the knee extension after double-bundle ACL reconstruction.

yes

### Human: Until now, the influence on knees with hyperextension has not been evaluated . Study evaluated whether double-bundle ACL reconstruction restricts extension in hypereextendable knees . The knee extension was 4°± 1.8° with the intact ACL and 4° ±  1.7° after reconstruction in the SB group (n.s) The limitation of the extension was significantly larger in the DB group (p = 0.013) The difference in AP translation before and after the reconstruction was not significantly different in 30° and 90° of flexion . Does anatomic double-bundle ACL reconstruction restrict knee extension in knees with hyperextension?### Assistant: Both single- and double-bundle ACL reconstruction techniques are capable of restoring knee anteroposterior and rotational stability. Double-bundle reconstructions significantly reduce knee extension in knees with hyperextension capability. Care must be taken when using double-bundle techniques in patients with knee hyperextension as this procedure may limit the knee extension after double-bundle ACL reconstruction.

Double-bundle reconstructions significantly reduce knee extension in knees with hyperextension capability . Care must be taken when using double-

Does acute fluoride poisoning alter myocardial cytoskeletal and AMPK signaling proteins in rats?

Male Wistar rats were treated with single oral doses of 45 and 90mg/kgFAcute F . The study found that increased oxidative stress, apoptosis and necrosis were implicated in acute fluoride (F) Our previous findings revealed that increased . stress, . apoptosis, . necrosis and . oxidative stress were implicated . in acute . fluoride . (Fluidine) was implicated in . acute fluoride in the study of male Wistar . rats . Male rats were . treated with . single oral dose of . 45 and . 90mg /kgFACute F

Taken together, these findings reveal that acute F

yes

### Human: Male Wistar rats were treated with single oral doses of 45 and 90mg/kgFAcute F . The study found that increased oxidative stress, apoptosis and necrosis were implicated in acute fluoride (F) Our previous findings revealed that increased . stress, . apoptosis, . necrosis and . oxidative stress were implicated . in acute . fluoride . (Fluidine) was implicated in . acute fluoride in the study of male Wistar . rats . Male rats were . treated with . single oral dose of . 45 and . 90mg /kgFACute F Does acute fluoride poisoning alter myocardial cytoskeletal and AMPK signaling proteins in rats?### Assistant: Taken together, these findings reveal that acute F

Taken together, these findings reveal that acute F is linked to acute F .

Does blockade of glycoprotein IIb/IIIa mediate the antithrombotic activity of butanol fraction of Actinostemma lobatum Maxim?

Actinostemma lobatum Maxim, a wildlife plant of Cucurbitaceae family, has been utilized for the prevention or treatment of cardiovascular diseases as a folk remedy in Korea . The study examined the effects of butanol fraction of Act in vitro and in . vitro and . in . vivo antithrombotic activity and possible mechanisms were . elucidated for the first time . oral administration of BFALM resulted in a dose-dependent increase of survival rates of mice with pulmonary thromboembolism .

These results suggest that BFALM may exert antithrombotic activity through inhibition of platelet aggregation via GP IIb/IIIa and thromboxane A(2) pathways, along with anticoagulatory activity through intrinsic and extrinsic pathways.

yes

### Human: Actinostemma lobatum Maxim, a wildlife plant of Cucurbitaceae family, has been utilized for the prevention or treatment of cardiovascular diseases as a folk remedy in Korea . The study examined the effects of butanol fraction of Act in vitro and in . vitro and . in . vivo antithrombotic activity and possible mechanisms were . elucidated for the first time . oral administration of BFALM resulted in a dose-dependent increase of survival rates of mice with pulmonary thromboembolism . Does blockade of glycoprotein IIb/IIIa mediate the antithrombotic activity of butanol fraction of Actinostemma lobatum Maxim?### Assistant: These results suggest that BFALM may exert antithrombotic activity through inhibition of platelet aggregation via GP IIb/IIIa and thromboxane A(2) pathways, along with anticoagulatory activity through intrinsic and extrinsic pathways.

BFALM may exert antithrombotic activity through inhibition of platelet aggregation via GP IIb/IIIa and throm

Are a new variation in the promoter region , the -604 C > T , and the Leu72Met polymorphism of the ghrelin gene associated with protection to insulin resistance?

Previous studies suggested polymorphisms in the coding region of the preproghrelin gene might modulate glucose-induced insulin secretion . We evaluated the association of a new variation, -604C>T, in the promoter region of ghrelin, of Leu72Met (247C>A) and of Gln90Leu (265A>T), with measures of insulin sensitivity in 1420 adult individuals . We observed a protective effect exerted by Met72 variant of the Leu 72Met SNP on insulin resistance .

Our observations suggest a protective role of the Met72 variant and of -604 T allele in modulating insulin resistance. These SNPs or an unknown functional variant in linkage disequilibrium could increase ghrelin levels and probably insulin sensitivity.

yes

### Human: Previous studies suggested polymorphisms in the coding region of the preproghrelin gene might modulate glucose-induced insulin secretion . We evaluated the association of a new variation, -604C>T, in the promoter region of ghrelin, of Leu72Met (247C>A) and of Gln90Leu (265A>T), with measures of insulin sensitivity in 1420 adult individuals . We observed a protective effect exerted by Met72 variant of the Leu 72Met SNP on insulin resistance . Are a new variation in the promoter region , the -604 C > T , and the Leu72Met polymorphism of the ghrelin gene associated with protection to insulin resistance?### Assistant: Our observations suggest a protective role of the Met72 variant and of -604 T allele in modulating insulin resistance. These SNPs or an unknown functional variant in linkage disequilibrium could increase ghrelin levels and probably insulin sensitivity.

SNPs or an unknown functional variant in linkage disequilibrium could increase ghrelin levels and probably insulin sensitivity .

Does iGF-1 from adipose-derived mesenchymal stem cells promote radioresistance of breast cancer cells?

AMSC supernatants promoted proliferation and radioresistance of breast cancer cells . Breast cancer cells could recruit AMSCs, especially after irradiation . IGF-1 derived from AMSC may be responsible for the radioresistant of breast cancers . AMSC cells could also recruit AMS cells after irradiated . Breast cancers could recruit the AMSC, especially post-irradiation, study finds . AMS supernatantants promoted proliferating and radiorersistance of cancer cells, according to study .

Our results suggest that AMSCs in the tumor microenvironment may affect the outcome of radiotherapy for breast cancer in vitro.

yes

### Human: AMSC supernatants promoted proliferation and radioresistance of breast cancer cells . Breast cancer cells could recruit AMSCs, especially after irradiation . IGF-1 derived from AMSC may be responsible for the radioresistant of breast cancers . AMSC cells could also recruit AMS cells after irradiated . Breast cancers could recruit the AMSC, especially post-irradiation, study finds . AMS supernatantants promoted proliferating and radiorersistance of cancer cells, according to study . Does iGF-1 from adipose-derived mesenchymal stem cells promote radioresistance of breast cancer cells?### Assistant: Our results suggest that AMSCs in the tumor microenvironment may affect the outcome of radiotherapy for breast cancer in vitro.

AMSCs in the tumor microenvironment may affect the outcome of radiotherapy for breast cancer in vitro .

Does five-region study found no evidence of undiagnosed type 2 diabetes in Swedish 11- to 13-year-olds?

The aim of this study was to estimate the prevalence of undiagnosed type 2 diabetes in overweight Swedish school children from 11 to 13 years of age . Of 1126 children with ISO-BMI ≥25, 24 (2.1%) had at least one HbA1c value ≥6.1% on two occasions . Three of the children had Hb A1c ≥6%. Three of them had a normal Hb a1c, and all the children with a normal OGTT .

In this cross-sectional, population-based screening study of a high-risk group of 11- to 13-year-old Swedish school children, we found no indication of undiagnosed diabetes or impaired glucose tolerance.

yes

### Human: The aim of this study was to estimate the prevalence of undiagnosed type 2 diabetes in overweight Swedish school children from 11 to 13 years of age . Of 1126 children with ISO-BMI ≥25, 24 (2.1%) had at least one HbA1c value ≥6.1% on two occasions . Three of the children had Hb A1c ≥6%. Three of them had a normal Hb a1c, and all the children with a normal OGTT . Does five-region study found no evidence of undiagnosed type 2 diabetes in Swedish 11- to 13-year-olds?### Assistant: In this cross-sectional, population-based screening study of a high-risk group of 11- to 13-year-old Swedish school children, we found no indication of undiagnosed diabetes or impaired glucose tolerance.

No indication of undiagnosed diabetes or impaired glucose tolerance, study finds . Cross-sectional, population-based screening study of a

Does arsenic trioxide modulate the central snail neuron action potential?

The effect of arsenic trioxide (As2O3) is unknown . The RP4 neuron generated spontaneous action potentials that were affected by the concentration-dependent effect of AsO3 . Extracellular application of 1 or 3 M As2O 3 decreased the frequency of spontaneously generated action potential . The BoPs elicited by 10mM As2 O3 were blocked when neurons were pretreated with phospholipase C (PLC) inhibitors (10 microM U73122 or 3mM neomycin)

As2O3 at 10 mM elicits BoPs in central snail neurons and this effect may relate to the PLC activity of the neuron, rather than protein kinase A activity, or calcium influxes of the neuron. As2O3 at higher concentration irreversibly abolishes the spontaneous action potentials of the neuron.

yes

### Human: The effect of arsenic trioxide (As2O3) is unknown . The RP4 neuron generated spontaneous action potentials that were affected by the concentration-dependent effect of AsO3 . Extracellular application of 1 or 3 M As2O 3 decreased the frequency of spontaneously generated action potential . The BoPs elicited by 10mM As2 O3 were blocked when neurons were pretreated with phospholipase C (PLC) inhibitors (10 microM U73122 or 3mM neomycin) Does arsenic trioxide modulate the central snail neuron action potential?### Assistant: As2O3 at 10 mM elicits BoPs in central snail neurons and this effect may relate to the PLC activity of the neuron, rather than protein kinase A activity, or calcium influxes of the neuron. As2O3 at higher concentration irreversibly abolishes the spontaneous action potentials of the neuron.

As2O3 at 10 Mnomeric eradently abolishes spontaneous action potentials of the neuron . As2

Does effect of resveratrol on experimental non-alcoholic fatty liver disease depend on severity of pathology and timing of treatment?

Resveratrol (RSV) prevents the development of steatosis in a number of experimental fatty liver (non-alcoholic fatty liver [NAFL] models) But clinical results on NASH are ambiguous . We found RSV metabolites but no parent RSV in serum or liver tissue, confirming low bioavailability . In NASH prevention, RSV reduced the accumulation of triglyceride in hepatic tissue (P < 0.01), while there was no effect on biochemical, histopathological, or transcriptional NASH changes .

These experimental findings suggest that a weak hepatic benefit of RSV treatment is seen in prevention of steatosis only.

yes

### Human: Resveratrol (RSV) prevents the development of steatosis in a number of experimental fatty liver (non-alcoholic fatty liver [NAFL] models) But clinical results on NASH are ambiguous . We found RSV metabolites but no parent RSV in serum or liver tissue, confirming low bioavailability . In NASH prevention, RSV reduced the accumulation of triglyceride in hepatic tissue (P < 0.01), while there was no effect on biochemical, histopathological, or transcriptional NASH changes . Does effect of resveratrol on experimental non-alcoholic fatty liver disease depend on severity of pathology and timing of treatment?### Assistant: These experimental findings suggest that a weak hepatic benefit of RSV treatment is seen in prevention of steatosis only.

These experimental findings suggest that a weak hepatic benefit of RSV treatment is seen in prevention of steatosis only .

Do intracellular oxidative stress and cadmium ions release induce cytotoxicity of unmodified cadmium sulfide quantum dots?

After-degradation QDs are more toxic than microsized CdS nanoparticles, especially at concentrations below 40 microg/ml . Microsized particles were simultaneously tested as a comparison . Nearly 20% of cadmium was released from QDs nanoparticles within 24h, which also accounts for QDs' toxicity . In the presence of NAC, cells are partially protected from CdsS QDs, but not from microsized particles, according to MTT . CdsQDs increase ROS by 20-30% over control levels, but they both deplete cellular GSH significantly at medium concentration of 20 microg

Intracellular ROS production, GSH depletion, and cadmium ions (Cd(2+)) release are possible mechanisms for CdS QDs' cytotoxicity. We also suggested that with QD concentration increasing, the principal toxicity mechanism changes from intracellular oxidative stress to Cd(2+) release.

yes

### Human: After-degradation QDs are more toxic than microsized CdS nanoparticles, especially at concentrations below 40 microg/ml . Microsized particles were simultaneously tested as a comparison . Nearly 20% of cadmium was released from QDs nanoparticles within 24h, which also accounts for QDs' toxicity . In the presence of NAC, cells are partially protected from CdsS QDs, but not from microsized particles, according to MTT . CdsQDs increase ROS by 20-30% over control levels, but they both deplete cellular GSH significantly at medium concentration of 20 microg Do intracellular oxidative stress and cadmium ions release induce cytotoxicity of unmodified cadmium sulfide quantum dots?### Assistant: Intracellular ROS production, GSH depletion, and cadmium ions (Cd(2+)) release are possible mechanisms for CdS QDs' cytotoxicity. We also suggested that with QD concentration increasing, the principal toxicity mechanism changes from intracellular oxidative stress to Cd(2+) release.

Intracellular ROS production, GSH depletion, and cadmium ions (Cd(2+) release are possible mechanisms for

Does direct thrombin inhibition appear to be a safe and effective anticoagulant for percutaneous bypass graft interventions?

Little is known concerning the use of direct thrombin inhibition during CABG intervention . Bivalirudin with GPIIb/IIIa blockade inhibition was used in two studies . No significant differences in the combined endpoint of death, myocardial infarction, urgent revascularization, or major bleeding when patients were treated with either heparin or bivalirulin . Minor bleeding was significantly reduced in patients treated with bivalrudin (14.8% vs. 22.7%)

CABG PCI using bivalirudin with provisional GPIIb/IIIa inhibition appears to provide similar safety and efficacy to heparin with GPIIb/IIIa inhibition.

yes

### Human: Little is known concerning the use of direct thrombin inhibition during CABG intervention . Bivalirudin with GPIIb/IIIa blockade inhibition was used in two studies . No significant differences in the combined endpoint of death, myocardial infarction, urgent revascularization, or major bleeding when patients were treated with either heparin or bivalirulin . Minor bleeding was significantly reduced in patients treated with bivalrudin (14.8% vs. 22.7%) Does direct thrombin inhibition appear to be a safe and effective anticoagulant for percutaneous bypass graft interventions?### Assistant: CABG PCI using bivalirudin with provisional GPIIb/IIIa inhibition appears to provide similar safety and efficacy to heparin with GPIIb/IIIa inhibition.

CABG PCI using bivalirudin with provisional GPIIb/IIIa inhibition appears to provide similar safety and efficacy to

Does ghrelin contribute to derangements of glucose metabolism induced by rapamycin in mice?

The effects of rapamycin on glucose metabolism were examined in mice receiving ghrelin receptor antagonist or with Ghsr1a gene knockout . Changes in GLUT4, c-Jun N-terminal kinase (JNK) and phosphorylated ribosomal protein S6 (pS6) were investigated by immunofluorescent staining or western blotting . The deterioration in glucose homeostasis was blocked by D: -Lys3-GHRP-6, a ghrehn receptor antagonist .

The current study demonstrates that ghrelin contributes to derangements of glucose metabolism induced by rapamycin via altering the content and translocation of GLUT4 in muscles.

yes

### Human: The effects of rapamycin on glucose metabolism were examined in mice receiving ghrelin receptor antagonist or with Ghsr1a gene knockout . Changes in GLUT4, c-Jun N-terminal kinase (JNK) and phosphorylated ribosomal protein S6 (pS6) were investigated by immunofluorescent staining or western blotting . The deterioration in glucose homeostasis was blocked by D: -Lys3-GHRP-6, a ghrehn receptor antagonist . Does ghrelin contribute to derangements of glucose metabolism induced by rapamycin in mice?### Assistant: The current study demonstrates that ghrelin contributes to derangements of glucose metabolism induced by rapamycin via altering the content and translocation of GLUT4 in muscles.

ghrelin contributes to derangements of glucose metabolism induced by rapamycin via altering the content and translocation of GLUT4

Do distinct bacterial colonization patterns of Escherichia coli subtypes associate with rheumatoid factor status in early inflammatory arthritis?

The aetiology of RA is unknown, but bacterial exposure to Escherichia coli, Proteus mirabilis and Klebsiella pneumoniae has been linked to disease pathogenesis . The strongest association was observed for RF(+) RA . RF(+) patients were more commonly colonized with phylogenetic Group D E. coli, whereas RF(-) patients had lower joint scores and inflammatory markers yet higher IgA anti-E. coli antibody responses . These individuals also had lower joints scores and higher inflammatory markers .

These studies link the type of colonizing bacteria in the gut and urine with the immune response (anti-bacterial and RF) in early-onset inflammatory arthritis and provide evidence for a role of the host-pathogen response in the aetiology of RF.

yes

### Human: The aetiology of RA is unknown, but bacterial exposure to Escherichia coli, Proteus mirabilis and Klebsiella pneumoniae has been linked to disease pathogenesis . The strongest association was observed for RF(+) RA . RF(+) patients were more commonly colonized with phylogenetic Group D E. coli, whereas RF(-) patients had lower joint scores and inflammatory markers yet higher IgA anti-E. coli antibody responses . These individuals also had lower joints scores and higher inflammatory markers . Do distinct bacterial colonization patterns of Escherichia coli subtypes associate with rheumatoid factor status in early inflammatory arthritis?### Assistant: These studies link the type of colonizing bacteria in the gut and urine with the immune response (anti-bacterial and RF) in early-onset inflammatory arthritis and provide evidence for a role of the host-pathogen response in the aetiology of RF.

These studies link the type of colonizing bacteria in the gut and urine with the immune response (anti-bacterial and RF) in

Does doxycycline reduce early neurologic impairment after cerebral arterial air embolism in the rabbit?

Previous studies indicate leukocytes play a role in the pathogenesis of cerebral arterial air embolism . Because doxycycline inhibits numerous leukocyte activities, the authors hypothesized doxy cycline would decrease neurologic impairment after cerebral air emblism . Animals were neurologically evaluated 4 h after anesthetized with methohexital on a scale of 0 (normal) to 99 (coma) points . At 4 h, animals had lesser impairment than animals that received saline (77 +/- 20; median, 81)

Doxycycline decreased electrophysiologic and neurologic abnormalities after cerebral air embolism. Because groups could be distinguished electrophysiologically as soon as 1 h after air embolism and because SSEP amplitude inversely correlated with neurologic impairment, doxycycline appears to inhibit a key early (approximately 1 h) process in the pathophysiology of cerebral air embolism.

yes

### Human: Previous studies indicate leukocytes play a role in the pathogenesis of cerebral arterial air embolism . Because doxycycline inhibits numerous leukocyte activities, the authors hypothesized doxy cycline would decrease neurologic impairment after cerebral air emblism . Animals were neurologically evaluated 4 h after anesthetized with methohexital on a scale of 0 (normal) to 99 (coma) points . At 4 h, animals had lesser impairment than animals that received saline (77 +/- 20; median, 81) Does doxycycline reduce early neurologic impairment after cerebral arterial air embolism in the rabbit?### Assistant: Doxycycline decreased electrophysiologic and neurologic abnormalities after cerebral air embolism. Because groups could be distinguished electrophysiologically as soon as 1 h after air embolism and because SSEP amplitude inversely correlated with neurologic impairment, doxycycline appears to inhibit a key early (approximately 1 h) process in the pathophysiology of cerebral air embolism.

Doxycycline decreased electrophysiologic and neurologic abnormalities after cerebral air embolism .

Are pLAC4 and beta-HCG mRNA levels altered in the maternal circulation of pregnancies with trisomy 21?

PAPP-A- and PLAC4 mRNAs are placentally produced proteins whose levels are altered in pregnancies with trisomy 21 . The levels of beta-HCG- and PAPPA- mRNA significantly correlated with the circulatory concentrations of the HCG protein . However, neither beta- HCG-- nor PLAC 4 mRNas show a significant difference between cases and controls, and the levels of PAPP A and HCG proteins were found to be significantly different in both cases .

Maternal blood levels of beta-HCG-, PLAC4- and PAPP-A mRNAs are not useful markers for the screening of pregnancies with trisomy 21 as their concentrations are either not significantly altered (beta-HCG and PLAC4) or too low to be detected (PAPP-A).

no

### Human: PAPP-A- and PLAC4 mRNAs are placentally produced proteins whose levels are altered in pregnancies with trisomy 21 . The levels of beta-HCG- and PAPPA- mRNA significantly correlated with the circulatory concentrations of the HCG protein . However, neither beta- HCG-- nor PLAC 4 mRNas show a significant difference between cases and controls, and the levels of PAPP A and HCG proteins were found to be significantly different in both cases . Are pLAC4 and beta-HCG mRNA levels altered in the maternal circulation of pregnancies with trisomy 21?### Assistant: Maternal blood levels of beta-HCG-, PLAC4- and PAPP-A mRNAs are not useful markers for the screening of pregnancies with trisomy 21 as their concentrations are either not significantly altered (beta-HCG and PLAC4) or too low to be detected (PAPP-A).

Maternal blood levels of beta-HCG-, PLAC4- and PAPP-A mRNAs are not useful markers for

Are self-reported and automatic cognitions associated with exercise behavior in cancer survivors?

Social-cognitive theory constructs such as outcome expectancies predict exercise behavior, but self-report measures have several limitations . We examined associations between implicit (automatic) cognitions and exercise behavior in endometrial cancer survivors . Exercise implicit attitudes and self-identification were prospectively associated with exercise self-efficacy only after adjustment for the corresponding self-reported measures and baseline selfefficacy . Study was a longitudinal study to examine predictors of exercise behavior among female cancer survivors who all received an exercise intervention .

Both self-reported cognitions and implicit cognitions may be useful to identify individuals at risk of failing to exercise. Individuals so identified might be provided with a different or more intensive intervention. The data also suggest cognitive targets for intervention. (PsycINFO Database Record

yes

### Human: Social-cognitive theory constructs such as outcome expectancies predict exercise behavior, but self-report measures have several limitations . We examined associations between implicit (automatic) cognitions and exercise behavior in endometrial cancer survivors . Exercise implicit attitudes and self-identification were prospectively associated with exercise self-efficacy only after adjustment for the corresponding self-reported measures and baseline selfefficacy . Study was a longitudinal study to examine predictors of exercise behavior among female cancer survivors who all received an exercise intervention . Are self-reported and automatic cognitions associated with exercise behavior in cancer survivors?### Assistant: Both self-reported cognitions and implicit cognitions may be useful to identify individuals at risk of failing to exercise. Individuals so identified might be provided with a different or more intensive intervention. The data also suggest cognitive targets for intervention. (PsycINFO Database Record

Self-reported cognitions and implicit cognitions may be useful to identify individuals at risk of failing to exercise . Individuals so identified might be

Does ziprasidone -- not haloperidol -- induce more de-novo neurogenesis of adult neural stem cells derived from murine hippocampus?

Strain-cell based study on the de-novo generation of beta-III-tubulin-positive neurons after treatment with the classic antipsychotic drug haloperidol or the second-generation antipsychosis (SGA) ziprasidone . Adult neural stem cells (ANSC) dissociated from the adult mouse hippocampus were expanded in cell culture with basic fibroblast growth factor (bFGF) and epidermal growth factor . ANSC differentiated upon withdrawal of EGF and bFGF .

Ziprasidone generated significantly more beta-III-tubulin-positive neurons than haloperidol during the differentiation of adult neural stem cells isolated from murine hippocampus (ANSC). We assume that this net increase in neurogenesis by ziprasidone relies on this drug's 5-HT1A receptor affinity, which is not present in the haloperidol molecule, since the inactivation by WAY100621 impeded this process. These data could possibly suggest a clinical relevance for studying antipsychotic drugs in the stem cell paradigm employed in this study.

yes

### Human: Strain-cell based study on the de-novo generation of beta-III-tubulin-positive neurons after treatment with the classic antipsychotic drug haloperidol or the second-generation antipsychosis (SGA) ziprasidone . Adult neural stem cells (ANSC) dissociated from the adult mouse hippocampus were expanded in cell culture with basic fibroblast growth factor (bFGF) and epidermal growth factor . ANSC differentiated upon withdrawal of EGF and bFGF . Does ziprasidone -- not haloperidol -- induce more de-novo neurogenesis of adult neural stem cells derived from murine hippocampus?### Assistant: Ziprasidone generated significantly more beta-III-tubulin-positive neurons than haloperidol during the differentiation of adult neural stem cells isolated from murine hippocampus (ANSC). We assume that this net increase in neurogenesis by ziprasidone relies on this drug's 5-HT1A receptor affinity, which is not present in the haloperidol molecule, since the inactivation by WAY100621 impeded this process. These data could possibly suggest a clinical relevance for studying antipsychotic drugs in the stem cell paradigm employed in this study.

Ziprasidone generated significantly more beta-III-tubulin-positive neurons than haloperidol . These data could possibly suggest

Does stimulus familiarity affect perceptual restoration in the European starling ( Sturnus vulgaris )?

European starlings were trained to report salient differences between successive sounds . The birds' response latency for discriminating between a stimulus pair is an indicator for the salience of the difference . For familiar motifs the birds showed a large perceptual distance if discriminating between song motifs that were muted for brief time periods and complete motifs . For unfamiliar motifs no such difference was observed. For familiar . motifs, birds showed no difference if the muted periods were filled with noise, the perceptual distance was reduced .

The results suggest that starlings are able to perceptually restore partly masked sounds and, similarly to humans, rely on prior experience. They may be a suitable model to study the mechanism underlying experience-dependent perceptual restoration.

yes

### Human: European starlings were trained to report salient differences between successive sounds . The birds' response latency for discriminating between a stimulus pair is an indicator for the salience of the difference . For familiar motifs the birds showed a large perceptual distance if discriminating between song motifs that were muted for brief time periods and complete motifs . For unfamiliar motifs no such difference was observed. For familiar . motifs, birds showed no difference if the muted periods were filled with noise, the perceptual distance was reduced . Does stimulus familiarity affect perceptual restoration in the European starling ( Sturnus vulgaris )?### Assistant: The results suggest that starlings are able to perceptually restore partly masked sounds and, similarly to humans, rely on prior experience. They may be a suitable model to study the mechanism underlying experience-dependent perceptual restoration.

Starlings are able to perceptually restore partly masked sounds and, similarly to humans, rely on prior experience . They may be a suitable

Do an update on lisdexamfetamine dimesylate for the treatment of attention deficit hyperactivity disorder?

The efficacy and safety of stimulants for the pharmacologic management of attention deficit hyperactivity disorder (ADHD) is well documented . The focus of this paper is the prodrug amphetamine stimulant, lisdexamfetamine (LDX) The review covers the proof-of-concept and later studies of LDX to describe its use to treat ADHD in pediatric and adult populations . A literature search and review was carried out using the PubMed database up to August 2012 . A review of the literature of LDx were carried out .

Clinical studies of LDX in children and adults with ADHD demonstrate its tolerability and its efficacy in reducing ADHD symptoms. Future research should be less restrictive in order to address some of the unmet needs in ADHD treatment. The inclusion of patients with ADHD and co-occurring mental health disorders and/or medical conditions is typically not studied in clinical trials nor is the prior ADHD treatment exposure of study participants. The preschool age population also is understudied in recently approved ADHD treatments such as LDX. Finally, how to approach the treatment of participants or first-degree relatives with a medical history or presence of substance use disorder presents an ongoing clinical challenge.

yes

### Human: The efficacy and safety of stimulants for the pharmacologic management of attention deficit hyperactivity disorder (ADHD) is well documented . The focus of this paper is the prodrug amphetamine stimulant, lisdexamfetamine (LDX) The review covers the proof-of-concept and later studies of LDX to describe its use to treat ADHD in pediatric and adult populations . A literature search and review was carried out using the PubMed database up to August 2012 . A review of the literature of LDx were carried out . Do an update on lisdexamfetamine dimesylate for the treatment of attention deficit hyperactivity disorder?### Assistant: Clinical studies of LDX in children and adults with ADHD demonstrate its tolerability and its efficacy in reducing ADHD symptoms. Future research should be less restrictive in order to address some of the unmet needs in ADHD treatment. The inclusion of patients with ADHD and co-occurring mental health disorders and/or medical conditions is typically not studied in clinical trials nor is the prior ADHD treatment exposure of study participants. The preschool age population also is understudied in recently approved ADHD treatments such as LDX. Finally, how to approach the treatment of participants or first-degree relatives with a medical history or presence of substance use disorder presents an ongoing clinical challenge.

Clinical studies of LDX in children and adults with ADHD demonstrate its tolerability and its efficacy in reducing ADHD symptoms . Future research should be

Is recording lead V ( 4 ) R associated to enhanced use of fibrinolytic therapy in acute myocardial infarction?

Lead V(4)R in inferior wall myocardial infarction is recognized as a sign of proximal occlusion of the right coronary artery with evolving right ventricular mycardia . Patients with lead V4R recorded were more likely to receive fibrinolytic therapy compared with patients without recording (65% vs 51%; P = .035) Patients with V4(4), along with age (P < .001) and previous myocardia infarctions (OR 2.2, 95% CI 1.3-3.5) correlated to use of reperfusion therapy .

Lead V(4)R was recorded in only half of patients with inferior ST-elevation myocardial infarction. Patients with V(4)R recorded were more likely to receive thrombolytic therapy than those without recording of the additional chest lead.

yes

### Human: Lead V(4)R in inferior wall myocardial infarction is recognized as a sign of proximal occlusion of the right coronary artery with evolving right ventricular mycardia . Patients with lead V4R recorded were more likely to receive fibrinolytic therapy compared with patients without recording (65% vs 51%; P = .035) Patients with V4(4), along with age (P < .001) and previous myocardia infarctions (OR 2.2, 95% CI 1.3-3.5) correlated to use of reperfusion therapy . Is recording lead V ( 4 ) R associated to enhanced use of fibrinolytic therapy in acute myocardial infarction?### Assistant: Lead V(4)R was recorded in only half of patients with inferior ST-elevation myocardial infarction. Patients with V(4)R recorded were more likely to receive thrombolytic therapy than those without recording of the additional chest lead.

Lead V(4)R was recorded in only half of patients with inferior ST-elevation myocardial infarction .

Does endogenous IL-17 play a significant role in the development of experimental murine allergic conjunctivitis?

IL-17 is needed for the Ag sensitization that results in murine allergic asthma, a Th2-mediated disease . Here, we aimed to investigate the role IL-16 plays in the development of murine experimental allergic conjunctivitis (EC) which is also a Th 2-mediated illness . EC was induced by adoptively transferring RW-primed splenocytes followed by challenge with RW-containing eye drops . Reciprocal adoptive transfer experiments also demonstrated that the IL-18 from both donor splenocyte and recipient mice is not involved in development of EC .

Endogenous IL-17 does not appear to play a significant role in the development of EC.

no

### Human: IL-17 is needed for the Ag sensitization that results in murine allergic asthma, a Th2-mediated disease . Here, we aimed to investigate the role IL-16 plays in the development of murine experimental allergic conjunctivitis (EC) which is also a Th 2-mediated illness . EC was induced by adoptively transferring RW-primed splenocytes followed by challenge with RW-containing eye drops . Reciprocal adoptive transfer experiments also demonstrated that the IL-18 from both donor splenocyte and recipient mice is not involved in development of EC . Does endogenous IL-17 play a significant role in the development of experimental murine allergic conjunctivitis?### Assistant: Endogenous IL-17 does not appear to play a significant role in the development of EC.

Endogenous IL-17 does not appear to play a significant role in the development of EC .

Are muscle atrophy , voluntary activation disturbances , and low serum concentrations of IGF-1 and IGFBP-3 associated with weakness in people with chronic stroke?

Insulinlike growth factor 1 and IGF binding protein 3 (IGFBP-3) have been described as biomarkers of neuromuscular performance in many conditions . No information about these biomarkers is available for people with chronic hemiparesis . The purpose of this study was to investigate possible factors involved in muscle weakness in people with muscle weakness post stroke . Knee flexor and extensor muscle volumes were measured using nuclear magnetic resonance imaging, dynamometry, and electromyography .

Low serum concentrations of IGF-1 and IGFBP-3, deficits in neuromuscular performance, selective muscle atrophy, and decreased agonist muscle activation were found in the group with chronic hemiparesis poststroke. Both hemorrhagic and ischemic stroke were considered, and the data reflect a chronic poststroke population with good function.

yes

### Human: Insulinlike growth factor 1 and IGF binding protein 3 (IGFBP-3) have been described as biomarkers of neuromuscular performance in many conditions . No information about these biomarkers is available for people with chronic hemiparesis . The purpose of this study was to investigate possible factors involved in muscle weakness in people with muscle weakness post stroke . Knee flexor and extensor muscle volumes were measured using nuclear magnetic resonance imaging, dynamometry, and electromyography . Are muscle atrophy , voluntary activation disturbances , and low serum concentrations of IGF-1 and IGFBP-3 associated with weakness in people with chronic stroke?### Assistant: Low serum concentrations of IGF-1 and IGFBP-3, deficits in neuromuscular performance, selective muscle atrophy, and decreased agonist muscle activation were found in the group with chronic hemiparesis poststroke. Both hemorrhagic and ischemic stroke were considered, and the data reflect a chronic poststroke population with good function.

Low serum concentrations of IGF-1 and IGFBP-3, deficits in neuromuscular performance, selective muscle atrophy, and

Are allogeneic Transplantation for Patients With Advanced Myelofibrosis : Splenomegaly and High Serum LDH Adverse Risk Factors for Successful Engraftment?

Thirty consecutive patients underwent hematopoietic stem cell transplantation for myelofibrosis (MF) at our institution . Splenomegaly was associated with poor neutrophil engraftment (SHR), platelet engraftments and platelet recovery . Increased levels of lactate dehydrogenase (LDH) were associated with high NRM mortality (NRM) and relapse rates (RFS) The 4-year overall survival (OS) and RFS were 44% (95% CI, 29%-67%) and 37% (94%) respectively .

We confirm curative potential, but high NRM of allogeneic transplant for advanced MF.

yes

### Human: Thirty consecutive patients underwent hematopoietic stem cell transplantation for myelofibrosis (MF) at our institution . Splenomegaly was associated with poor neutrophil engraftment (SHR), platelet engraftments and platelet recovery . Increased levels of lactate dehydrogenase (LDH) were associated with high NRM mortality (NRM) and relapse rates (RFS) The 4-year overall survival (OS) and RFS were 44% (95% CI, 29%-67%) and 37% (94%) respectively . Are allogeneic Transplantation for Patients With Advanced Myelofibrosis : Splenomegaly and High Serum LDH Adverse Risk Factors for Successful Engraftment?### Assistant: We confirm curative potential, but high NRM of allogeneic transplant for advanced MF.

We confirm curative potential, but high NRM of allogeneic transplant for advanced MF .

Does pen ch 13 major fungal allergen decrease CD44 expression in human bronchial epithelial cells?

Pen ch 13 is an alkaline serine protease major allergen from Penicilliumchrysogenum . CD44 adhesion molecules play important roles in resolving lung inflammation and repairing epithelial damages during bronchial asthma . Pen ch . 13 dose-dependently down-regulates CD44 expression in 16HBE14o- cells . In addition, the decrease can be abolished by pre-treating Pen ch. 13 with a serine . protease inhibitor, phenylmethyl-sulfonyl fluoride .

Pen ch 13 allergen down-regulated CD44 protein expression in airway epithelial cells. It may contribute to atopic asthma by influencing the resolution of lung inflammation and prolonging the repair response of damaged bronchial epithelial cells.

yes

### Human: Pen ch 13 is an alkaline serine protease major allergen from Penicilliumchrysogenum . CD44 adhesion molecules play important roles in resolving lung inflammation and repairing epithelial damages during bronchial asthma . Pen ch . 13 dose-dependently down-regulates CD44 expression in 16HBE14o- cells . In addition, the decrease can be abolished by pre-treating Pen ch. 13 with a serine . protease inhibitor, phenylmethyl-sulfonyl fluoride . Does pen ch 13 major fungal allergen decrease CD44 expression in human bronchial epithelial cells?### Assistant: Pen ch 13 allergen down-regulated CD44 protein expression in airway epithelial cells. It may contribute to atopic asthma by influencing the resolution of lung inflammation and prolonging the repair response of damaged bronchial epithelial cells.

Pench 13 allergen down-regulated CD44 protein expression in airway epithelial cells . It may contribute to atopic asthma

Is satisfying parents ' preferences with regard to various models of postnatal care cost-minimizing?

Staff costs were calculated for various models of postnatal care in Sweden . The proportion of mothers receiving care at the maternity ward crucially influences the total costs . The staff costs differed significantly between the models, ranging from US$448 000 to US$778 000 per 1500 mother-child dyads . One of the models was based on answers from 342 parents who specified their preferences with regard to post-natal care for another birth in the event of another birth . One model was based upon answers from parents who provided their preferences .

Because the parents' preferences with regard to postnatal care created one of the most cost-minimizing care models in the study, it would be possible to better meet parent's desires and, at the same time, reduce costs, without increasing risks in comparison with the early discharge program.

yes

### Human: Staff costs were calculated for various models of postnatal care in Sweden . The proportion of mothers receiving care at the maternity ward crucially influences the total costs . The staff costs differed significantly between the models, ranging from US$448 000 to US$778 000 per 1500 mother-child dyads . One of the models was based on answers from 342 parents who specified their preferences with regard to post-natal care for another birth in the event of another birth . One model was based upon answers from parents who provided their preferences . Is satisfying parents ' preferences with regard to various models of postnatal care cost-minimizing?### Assistant: Because the parents' preferences with regard to postnatal care created one of the most cost-minimizing care models in the study, it would be possible to better meet parent's desires and, at the same time, reduce costs, without increasing risks in comparison with the early discharge program.

Parents' preferences with regard to postnatal care created one of the most cost-minimizing care models in the study . Early discharge

Is tCF7L2 rs7903146 polymorphism associated with gastric cancer : A case-control study in the Venezuelan population?

To explore the association between TCF7L2 single nucleotide polymorphisms (SNPs) and gastric cancer risk in Venezuelan patients, we performed a case-control study . Carriers of the TCF 7L2 SNP (rs12255372) had a significantly increased risk of moderate/well differentiated gastric cancers . We did not find association between the SNP and the susceptibility of developing gastric . cancer . risk of developing cancer was not found to be linked to the risk factor of developing the disease .

TCF7L2 rs7903146 polymorphism is associated with gastric cancer risk in the Venezuelan population, and could be related to determine the degree of differentiation of tumor cells.

yes

### Human: To explore the association between TCF7L2 single nucleotide polymorphisms (SNPs) and gastric cancer risk in Venezuelan patients, we performed a case-control study . Carriers of the TCF 7L2 SNP (rs12255372) had a significantly increased risk of moderate/well differentiated gastric cancers . We did not find association between the SNP and the susceptibility of developing gastric . cancer . risk of developing cancer was not found to be linked to the risk factor of developing the disease . Is tCF7L2 rs7903146 polymorphism associated with gastric cancer : A case-control study in the Venezuelan population?### Assistant: TCF7L2 rs7903146 polymorphism is associated with gastric cancer risk in the Venezuelan population, and could be related to determine the degree of differentiation of tumor cells.

TCF7L2 rs7903146 polymorphism is associated with gastric cancer risk in the Venezuelan population . It could be related

Is porcine cytomegalovirus infection associated with early rejection of kidney grafts in a pig to baboon xenotransplantation model?

Recent survivals of pig-to-baboon kidney xenotransplants have been markedly shorter than the graft survivals we previously reported . High levels of porcine cytomegalovirus (pCMV) in one of the rejected xenografts led us to evaluate whether this reduction in graft survival might be because of the inadvertent introduction of pCMV into our α1,3-galactosyltransferase gene knockout swine herd . Three prospective pig-To-Baboon renal transplants using kidneys from swine delivered by cesarean section (C-section) and raised in isolation were likewise analyzed .

Decreased survivals of α1,3-galactosyltransferase gene knockout renal xenografts in this laboratory correlate temporally with latent pCMV in the donor animals and pCMV in the rejected xeno-kidneys. Transmission of pCMV to swine offspring may be avoided by C-section delivery and scrupulous isolation of donor animals.

yes

### Human: Recent survivals of pig-to-baboon kidney xenotransplants have been markedly shorter than the graft survivals we previously reported . High levels of porcine cytomegalovirus (pCMV) in one of the rejected xenografts led us to evaluate whether this reduction in graft survival might be because of the inadvertent introduction of pCMV into our α1,3-galactosyltransferase gene knockout swine herd . Three prospective pig-To-Baboon renal transplants using kidneys from swine delivered by cesarean section (C-section) and raised in isolation were likewise analyzed . Is porcine cytomegalovirus infection associated with early rejection of kidney grafts in a pig to baboon xenotransplantation model?### Assistant: Decreased survivals of α1,3-galactosyltransferase gene knockout renal xenografts in this laboratory correlate temporally with latent pCMV in the donor animals and pCMV in the rejected xeno-kidneys. Transmission of pCMV to swine offspring may be avoided by C-section delivery and scrupulous isolation of donor animals.

PCMV may be avoided by C-section delivery and scrupulous isolation of donor animals . Transmission of pCMV to swine

Does microRNA-212 inhibit osteosarcoma cells proliferation and invasion by down-regulation of Sox4?

MicroRNAs have been identified in the development and progression of osteosarcoma . MiR-212 mimics were introduced into MG63 and U2OS cells . Introduction of miR-2012 mimics into MG64 and U3OS cells inhibited cell proliferation and invasion . MiRNAs were also found to inhibit tumor growth in the nude mice by overexpression of the sex-determining region Y-box 4 (Sox4) is a target gene of miRNA-212 .

The miR-212/Sox4 interaction plays an important role of in the osteosarcoma progression.

yes

### Human: MicroRNAs have been identified in the development and progression of osteosarcoma . MiR-212 mimics were introduced into MG63 and U2OS cells . Introduction of miR-2012 mimics into MG64 and U3OS cells inhibited cell proliferation and invasion . MiRNAs were also found to inhibit tumor growth in the nude mice by overexpression of the sex-determining region Y-box 4 (Sox4) is a target gene of miRNA-212 . Does microRNA-212 inhibit osteosarcoma cells proliferation and invasion by down-regulation of Sox4?### Assistant: The miR-212/Sox4 interaction plays an important role of in the osteosarcoma progression.

miR-212/Sox4 interaction plays an important role of in osteosarcoma progression .